Abstract
A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.
Keywords: Cell migration, fibronectin, gradient, haptotaxis, microvascular endothelial cell, morphology, acidic fibroblast growth factor, aFGF, LY294002
Combinatorial Chemistry & High Throughput Screening
Title: Haptotactic Gradients for Directed Cell Migration: Stimulation and Inhibition Using Soluble Factors
Volume: 12 Issue: 6
Author(s): Jason T. Smith, Donghwan H. Kim and William M. Reichert
Affiliation:
Keywords: Cell migration, fibronectin, gradient, haptotaxis, microvascular endothelial cell, morphology, acidic fibroblast growth factor, aFGF, LY294002
Abstract: A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.
Export Options
About this article
Cite this article as:
Smith T. Jason, Kim H. Donghwan and Reichert M. William, Haptotactic Gradients for Directed Cell Migration: Stimulation and Inhibition Using Soluble Factors, Combinatorial Chemistry & High Throughput Screening 2009; 12 (6) . https://dx.doi.org/10.2174/138620709788681871
DOI https://dx.doi.org/10.2174/138620709788681871 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Interleukin-10: An Anti-Inflammatory and Immunosuppressive Cytokine in the Normal and Pathological Immune Response
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Nonsteroidal Aromatase Inhibitors for the Treatment of Breast Cancer: An Update
Anti-Cancer Agents in Medicinal Chemistry Immunological Aspects of the Prostate Gland and Related Diseases
Current Immunology Reviews (Discontinued) From Bortezomib to other Inhibitors of the Proteasome and Beyond
Current Pharmaceutical Design Transfection of CXCR-4 Using Microbubble-Mediated Ultrasound Irradiation and Liposomes Improves the Migratory Ability of Bone Marrow Stromal Cells
Current Gene Therapy Recent Findings on the Application of Toll-like Receptors Agonists in Cancer Therapy
Current Medicinal Chemistry Gene Therapy Strategies in Prostate Cancer
Current Gene Therapy Targeting the PI3K/AKT/mTOR Signaling Pathway in Medulloblastoma
Current Molecular Medicine Non-Surgical Treatment Options for Symptomatic Uterine Leiomyomas
Current Women`s Health Reviews Epigenetic Multiple Modulators
Current Topics in Medicinal Chemistry Overcoming the Hurdles of Tumor Immunity by Targeting Regulatory Pathways in Innate and Adaptive Immune Cells
Current Pharmaceutical Design Antioxidant Therapy in Alzheimers Disease: Theory and Practice
Mini-Reviews in Medicinal Chemistry Targeting ADAM17 Sheddase Activity in Cancer
Current Drug Targets Targeted Therapies in Non-Small Cell Lung Cancer: Proven Concepts and Unfulfilled Promises.
Current Cancer Drug Targets Peptide-Based Anticancer Vaccines: Recent Advances and Future Perspectives
Current Medicinal Chemistry Peroxisome Proliferator Activated Receptor α Ligands as Anticancer Drugs Targeting Mitochondrial Metabolism
Current Pharmaceutical Biotechnology Cisplatin Properties in a Nanobiotechnological Approach to Cancer: A Mini-Review
Current Cancer Drug Targets Cancer Stem Cells: How can we Target them?
Current Medicinal Chemistry A Series of 2,4(1H,3H)-Quinazolinedione Derivatives: Synthesis and Biological Evaluation as Potential Anticancer Agents
Letters in Drug Design & Discovery Anti-Cancer Therapeutic Approaches Based on Intracellular and Extracellular Heat Shock Proteins
Current Medicinal Chemistry