Abstract
Carbohydrates exhibit many physiologically and pharmacologically important activities, yet their complicated structure and sequence pose major analytical challenges. Although their structural complexity makes analysis of carbohydrate difficult, mass spectrometry (MS) with high sensitivity, resolution and accuracy has become a vital tool in many applications related to analysis of carbohydrates or oligosaccharides. This application is essentially based on soft ionization technique which facilitates the ionization and vaporization of large, polar and thermally labile biomolecules. Electrospray- ionization (ESI), one of the soft ionization technique, tandem MS has been used in the sequencing of peptides, proteins, lipids, nucleic acids and more recently carbohydrates. The development of the ESI and tandem MS has begun to make carbohydrate analysis more routine. This review will focus on the application of the ESI tandem MS for the sequence analysis of native oligosaccharides, including neutral saccharides with multiple linkages, and the uronic acid polymers, alginate and glycosaminoglycans structures containing epimers.
Keywords: ESI tandem MS, Neutral oligosaccharide, Uronic acid oligosaccharides, Glycosaminoglycan oligosaccharides, Sequence
Current Analytical Chemistry
Title: Sequence Analysis of Native Oligosaccharides Using Negative ESI Tandem MS
Volume: 5 Issue: 3
Author(s): Zhenqing Zhang and Robert J. Linhardt
Affiliation:
Keywords: ESI tandem MS, Neutral oligosaccharide, Uronic acid oligosaccharides, Glycosaminoglycan oligosaccharides, Sequence
Abstract: Carbohydrates exhibit many physiologically and pharmacologically important activities, yet their complicated structure and sequence pose major analytical challenges. Although their structural complexity makes analysis of carbohydrate difficult, mass spectrometry (MS) with high sensitivity, resolution and accuracy has become a vital tool in many applications related to analysis of carbohydrates or oligosaccharides. This application is essentially based on soft ionization technique which facilitates the ionization and vaporization of large, polar and thermally labile biomolecules. Electrospray- ionization (ESI), one of the soft ionization technique, tandem MS has been used in the sequencing of peptides, proteins, lipids, nucleic acids and more recently carbohydrates. The development of the ESI and tandem MS has begun to make carbohydrate analysis more routine. This review will focus on the application of the ESI tandem MS for the sequence analysis of native oligosaccharides, including neutral saccharides with multiple linkages, and the uronic acid polymers, alginate and glycosaminoglycans structures containing epimers.
Export Options
About this article
Cite this article as:
Zhang Zhenqing and Linhardt J. Robert, Sequence Analysis of Native Oligosaccharides Using Negative ESI Tandem MS, Current Analytical Chemistry 2009; 5 (3) . https://dx.doi.org/10.2174/157341109788680291
DOI https://dx.doi.org/10.2174/157341109788680291 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Biogeochemistry: global environmental research and management
his issue focuses on the bio-pathways of trace elements (Ni, Co, Cr, Mn, Cd, Pb, Zn and REE) in soil, plant and atmospheric systems. Specific interest in the application of phytotechnologies that utilise hyperaccumulator plants (agro/phytomining). On the basis of understanding the migration and transformation of trace elements in ecosystem, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Persistent But Not Replicating HIV-1 Cell-Associated DNA in Semen of Long-Term ART Experienced Men
Current HIV Research Sex Differences and Emerging New Risk Factors for Atherosclerosis and Its Thrombotic Complications
Current Pharmaceutical Design Cilostazol in the Management of Atherosclerosis
Current Vascular Pharmacology Hypericin Lights Up the Way for the Potential Treatment of Nasopharyngeal Cancer by Photodynamic Therapy
Current Clinical Pharmacology Drug Eluting Stents: Friend or Foe? A Review of Cellular Mechanisms Behind the Effects of Paclitaxel and Sirolimus Eluting Stents
Current Drug Metabolism Cardiovascular Pharmacogenetics of Anti-Thrombotic Agents and Non-Steroidal Anti-Inflammatory Drugs
Current Molecular Medicine Cellular Senescence in Ageing, Age-Related Disease and Longevity
Current Vascular Pharmacology Autoimmune (Auto-inflammatory) Syndrome Induced by Adjuvants (ASIA) – Animal Models as a Proof of Concept
Current Medicinal Chemistry Arachidonic Acid Cascade Enzyme Inhibition and Cancer
Current Enzyme Inhibition Antifungal Drug Discovery through the Study of Invertebrate Model Hosts
Current Medicinal Chemistry Fluoroquinolones: Blessings Or Curses
Current Drug Targets Cardiovascular Effects of Losartan and Its Relevant Clinical Application
Current Medicinal Chemistry Receptor Tyrosine Kinases are Signaling Intermediates of G Protein- Coupled Receptors
Current Pharmaceutical Design The Role of Oxidative Stress in the Pathogenesis of Type 2 Diabetes Mellitus Micro- and Macrovascular Complications: Avenues for a Mechanistic-Based Therapeutic Approach
Current Diabetes Reviews Mechanisms Governing Lentivirus Integration Site Selection
Current Gene Therapy Breast-Milk Characteristics Protecting Against Allergy
Endocrine, Metabolic & Immune Disorders - Drug Targets Brain-gut Axis and Pentadecapeptide BPC 157: Theoretical and Practical Implications
Current Neuropharmacology Catching the Therapeutic Window of Opportunity in Early Crohn`s Disease
Current Drug Targets The Potential Effect of Carvedilol Against Osteoporosis in Ovariectomized Rats
Current Drug Therapy Electrotransfer into Skeletal Muscle for Protein Expression
Current Gene Therapy