Stress-Free Chromatography: Affinity Chromatography

Title: Stress-Free Chromatography: Affinity Chromatography

Volume: 10 Issue: 4

Author(s): Tsutomu Arakawa, Yoshiko Kita, Haruna Sato and Daisuke Ejima

Affiliation:

Abstract: A number of approaches are available in minimizing aggregation of the final protein products. This chapter describes one such approach, i.e., an attempt to avoid stressful conditions that may eventually lead to protein aggregation. Affinity chromatography uses specific interaction between protein to be purified and ligand attached to the column. Due to high affinity, dissociation of such interaction and hence elution often require harsh solvent conditions. Ion exchange and hydrophobic interaction chromatography also pose certain stressful conditions on proteins. Here we describe development of mild elution buffer using arginine. This chapter covers Protein-A, dye, Protein-A mimetic and antigen affinity chromatography.

Cite this article as:

Arakawa Tsutomu, Kita Yoshiko, Sato Haruna and Ejima Daisuke, Stress-Free Chromatography: Affinity Chromatography, Current Pharmaceutical Biotechnology 2009; 10 (4) . https://dx.doi.org/10.2174/138920109788488969