Abstract
In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.
Keywords: Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS
Current Analytical Chemistry
Title: Bottom-Up Proteomics
Volume: 5 Issue: 2
Author(s): Andrea Armirotti
Affiliation:
Keywords: Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS
Abstract: In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.
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Cite this article as:
Armirotti Andrea, Bottom-Up Proteomics, Current Analytical Chemistry 2009; 5 (2) . https://dx.doi.org/10.2174/157341109787846117
DOI https://dx.doi.org/10.2174/157341109787846117 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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