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Current Analytical Chemistry

Editor-in-Chief

ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Bottom-Up Proteomics

Author(s): Andrea Armirotti

Volume 5, Issue 2, 2009

Page: [116 - 130] Pages: 15

DOI: 10.2174/157341109787846117

Price: $65

Abstract

In this work, the “bottom-up” protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.

Keywords: Proteomics, BOTTOM UP PROCESS, MALDI, LC-MS


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