Nitric Dioxide as Biologically Important Radical and its Role in Molecular Mechanism of Pancreatic Inflammation

Aleksandra      Dabrowska; Dagmara      Jacewicz; Agnieszka      Chylewska; Michal      Szkatula; Narcyz      Knap; Jolanta      Kubasik-Juraniec; Michal      Wozniak; Lech      Chmurzynski

Abstract

This review addresses a few vital issues pertaining to the chemical aspect of inflammatory stress mediated by • NO2. Consequently, in this paper we have presented a short description of different instrumental methods for the detection of nitrogen dioxide ( • NO2) in biological material and have demonstrated new instrumental method focusing on the reaction between an • NO2 and the two novel coordination ions of cis-[Cr(C2O4)(L-L)(OH2)2]+ [with L-L denoting either methyl 3-amino-2,3-dideoxy-α-D-arabino-hexopyranoside (AaraNH2) or methyl 3-amino-2,3-dideoxy-β-D-arabinohexopyranoside (BaraNH2)] studied by the stopped-flow technique. In order to investigate the biochemistry of • NO2 mediated inflammatory stress, we developed our method for specific detection of this radical in biological samples from pancreatic extracts and managed to identify • NO2 as an agent nitrating membrane localized proteins, as well as a selective oxidant of critical SH groups within membrane buried proteins. We have proved that the method is applicable to • NO2 assessment in tissue samples from rats with acute pancreatitis induced by L – arginine, where oxidative and nitrosative stress are supposed to play a key role in the pathomechanism of the disease.

Keywords: Nitrogen dioxide radical detection, New coordination biosensors, AaraNH2 and BaraNH2 as bioligands, Acute pancreatitis, L-arginine, 4-OH-TEMPO

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