Abstract
In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174° , we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture environment for experimental assessment of glial structure and function.
Keywords: Alkyl ketene dimer (AKD), C6 glioma cell, cAMP, cytoskeleton, fractal dimension
Current Nanoscience
Title: Morphology of C6 Glioma Cells on a Water-Repellent Fractal Alkyl Ketene Dimer Surface
Volume: 4 Issue: 3
Author(s): Wei-wei Hu, Hu Yan, Naoko Birukawa, Masashi Abe, Etsuro Ito, Kaoru Tsujiic, Zhong Chen, Akihisa Urano and Kaoru Tsujii
Affiliation:
Keywords: Alkyl ketene dimer (AKD), C6 glioma cell, cAMP, cytoskeleton, fractal dimension
Abstract: In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174° , we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture environment for experimental assessment of glial structure and function.
Export Options
About this article
Cite this article as:
Hu Wei-wei, Yan Hu, Birukawa Naoko, Abe Masashi, Ito Etsuro, Tsujiic Kaoru, Chen Zhong, Urano Akihisa and Tsujii Kaoru, Morphology of C6 Glioma Cells on a Water-Repellent Fractal Alkyl Ketene Dimer Surface, Current Nanoscience 2008; 4 (3) . https://dx.doi.org/10.2174/157341308785161118
DOI https://dx.doi.org/10.2174/157341308785161118 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Design and Application of Magnetic-Based Theranostic Nanoparticle Systems
Recent Patents on Biomedical Engineering (Discontinued) Anti-inflammatory and Immune Therapy for Alzheimers Disease: Current Status and Future Directions
Current Neuropharmacology Natural Molecules as Talented Inhibitors of Nucleotide Pyrophosphatases/ Phosphodiesterases (PDEs)
Current Topics in Medicinal Chemistry Cdc25A Protein Phosphatase: A Therapeutic Target for Liver Cancer Therapies
Anti-Cancer Agents in Medicinal Chemistry Assessment In Vitro of a Novel Therapeutic Strategy for Glioma, Combining Herpes Simplex Virus HSV1716-mediated Oncolysis with Gene Transfer and Targeted Radiotherapy
Medicinal Chemistry Therapeutic Potential of Curcumin in the Treatment of Glioblastoma Multiforme
Current Pharmaceutical Design Voltage-Dependent Potassium Channels Kv1.3 and Kv1.5 in Human Cancer
Current Cancer Drug Targets Implications of PEGylation of Carbon Nanotubes for Central Nervous System Bioavailability
CNS & Neurological Disorders - Drug Targets Modulation of Nitric Oxide Pathway by Multiligands/RAGE Axis: A Crossing Point on the Road to Microvascular Complication in Diabetes
Current Enzyme Inhibition Immune Response to Herpes Simplex Virus and γ134.5 Deleted HSV Vectors
Current Gene Therapy Resveratrol Counteracts Hypoxia-Induced Gastric Cancer Invasion and EMT through Hedgehog Pathway Suppression
Anti-Cancer Agents in Medicinal Chemistry Namitecan: a Hydrophilic Camptothecin with a Promising Preclinical Profile
Current Medicinal Chemistry AZT: An Old Drug with New Perspectives
Current Clinical Pharmacology Pharmaceutical Targeting of the Brain
Current Pharmaceutical Design Pharmacological Modulation of Caspase Activation
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents In Vitro Evidence for Competitive TSPO Binding of the Imaging Biomarker Candidates Vinpocetine and Two Iodinated DAA1106 Analogues in Post Mortem Autoradiography Experiments on Whole Hemisphere Human Brain Slices
Current Radiopharmaceuticals Advances Towards The Discovery of GPR55 Ligands
Current Medicinal Chemistry ADAM-Integrin Interactions: Potential Integrin Regulated Ectodomain Shedding Activity
Current Pharmaceutical Design Targeting the L-Arginine-Nitric Oxide Pathway for Cancer Treatment
Current Pharmaceutical Design Pituitary Adenylate Cyclase Activating Polypeptide: A Potential Neuroprotective Peptide
Current Pharmaceutical Design