Abstract
Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. More recently, absolute or stoichiometric quantification of proteome is becoming feasible, in particular, with the development of strategies with isotope-labeled standards composed of concatenated peptides. On the other hand, remarkable progress has been also made in label-free quantification methods based on the number of identified peptides. Here we review these mass spectrometry-based approaches for absolute quantification of proteome and discuss their implications.
Keywords: Quantitative proteomics, mass spectrometry, absolute quantification, stable isotope labeling, label-free
Current Genomics
Title: Mass Spectrometry-Based Approaches Toward Absolute Quantitative Proteomics
Volume: 9 Issue: 4
Author(s): Keiji Kito and Takashi Ito
Affiliation:
Keywords: Quantitative proteomics, mass spectrometry, absolute quantification, stable isotope labeling, label-free
Abstract: Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. More recently, absolute or stoichiometric quantification of proteome is becoming feasible, in particular, with the development of strategies with isotope-labeled standards composed of concatenated peptides. On the other hand, remarkable progress has been also made in label-free quantification methods based on the number of identified peptides. Here we review these mass spectrometry-based approaches for absolute quantification of proteome and discuss their implications.
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Cite this article as:
Kito Keiji and Ito Takashi, Mass Spectrometry-Based Approaches Toward Absolute Quantitative Proteomics, Current Genomics 2008; 9 (4) . https://dx.doi.org/10.2174/138920208784533647
DOI https://dx.doi.org/10.2174/138920208784533647 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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