Abstract
Different fractionation steps have been developed to reduce the complexity of serum proteome and to allow the detection and the identification of single proteins. The nature of fractionation (performed either on denatured or native proteins), the efficiency of recovery, the capacity of directly identifying proteins or protein panels, the possibility of associating to other laboratory techniques influence the choice of the methods to be used in different experimental and clinical settings. In this review, the main fractionation techniques (such as electrophoresis, SELDI and liquid chromatography) are described for proteomic studies in clinical field, and their advantages and disadvantages are discussed on the basis of our experience. In particular, liquid-liquid fractionation performed by PF2D is discussed in detail because of its partial automation, its ability to improve mass spectrometry analysis of serum proteins and its potential application in clinical studies.
Keywords: Serum, Proteomics, Fractionation, Clinical, Liquid Chromatography
Current Pharmaceutical Analysis
Title: Fractionation Techniques Improve the Proteomic Analysis of Human Serum
Volume: 4 Issue: 2
Author(s): Pierluigi Mauri, Andrea Petretto, Davide Cuccabita, Fabrizio Basilico, Dario Di Silvestre, Isabella Levreri and Giovanni Melioli
Affiliation:
Keywords: Serum, Proteomics, Fractionation, Clinical, Liquid Chromatography
Abstract: Different fractionation steps have been developed to reduce the complexity of serum proteome and to allow the detection and the identification of single proteins. The nature of fractionation (performed either on denatured or native proteins), the efficiency of recovery, the capacity of directly identifying proteins or protein panels, the possibility of associating to other laboratory techniques influence the choice of the methods to be used in different experimental and clinical settings. In this review, the main fractionation techniques (such as electrophoresis, SELDI and liquid chromatography) are described for proteomic studies in clinical field, and their advantages and disadvantages are discussed on the basis of our experience. In particular, liquid-liquid fractionation performed by PF2D is discussed in detail because of its partial automation, its ability to improve mass spectrometry analysis of serum proteins and its potential application in clinical studies.
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Mauri Pierluigi, Petretto Andrea, Cuccabita Davide, Basilico Fabrizio, Silvestre Di Dario, Levreri Isabella and Melioli Giovanni, Fractionation Techniques Improve the Proteomic Analysis of Human Serum, Current Pharmaceutical Analysis 2008; 4 (2) . https://dx.doi.org/10.2174/157341208784246297
DOI https://dx.doi.org/10.2174/157341208784246297 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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