Generic placeholder image

Current Organic Chemistry

Editor-in-Chief

ISSN (Print): 1385-2728
ISSN (Online): 1875-5348

Replicating DNA Differently

Author(s): Zheng-yun J. Zhan, Jingdong Ye, Xiaoyu Li and David G. Lynn

Volume 5, Issue 8, 2001

Page: [885 - 902] Pages: 18

DOI: 10.2174/1385272013375085

Price: $65

Abstract

The replication reaction provides the molecular source for mutation, selection and evolution in biology. This reaction also requires two additional stages, transcription and translation, functioning cooperatively, to construct the materials necessary to transfer the genome accurately to progeny. These three stages together constitute biologys Central Dogma, a term that highlights their presence in all autonomous replicating organisms. The template-directed synthesis that underpins each of these stages is dependent on molecular complementarity and the energies of self-assembly, suggesting it might now be possible to borrow from the structural tool set of biology to explore, diversify and even extend these reactions into different structural skeletons. Here we demonstrate, using simple ligation reactions, that it is conceptually possible to simplify the Central Dogma to a two-stage DNA replication process. Through this system DNA sequence information can be read into different molecular skeletons, s keletons with unique catalytic properties, that when extended, are amenable to mutation, selection, and evolution of desired function.

Keywords: replication reaction, mutation, Chemoselectivity of Reductants, Information Transfer, 5-O-Trityl-3-O-TBS-Thymidine, 3-O-TBS-Thymidine


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy