Abstract
In boranophosphate-oligodeoxynucleosides (BH 3- -ODN) a borane group replaces one of the two non-bridging oxygen atoms in the phosphodiester backbone of the naturally occurring congener. The chemical and biophysical properties of BH 3 - -ODN are reviewed, as are their interactions with enzymes such as DNA polymerases, exo- and endonucleases, and ribonuclease H. Three approaches to synthesis of BH 3- -ODN are described in solution, on solid supports, and by template directed enzymatic polymerization of appropriately modified nucleoside triphosphates. Comparisons are made to other members of the family of phosphorus modified nucleic acids, the phosphorothioates and methylphosphonates. The potential applications of boranophosphate modified compounds to antisense therapeutics and DNA sequencing are discussed.
Keywords: Boranophosphates, Natural Phosphodiesters, enzymatic polymerization, oligodeoxy nucleoside phosphorothioates, methylphosphonates, phosphoramidates, phosphoro fluoridates, F-ODN, Antisense Agents, Phosphodiester Analogs
Current Medicinal Chemistry
Title: Boranophosphates as Mimics of Natural Phosphodiesters in DNA
Volume: 8 Issue: 10
Author(s): Jack S. Summers and Barbara Ramsay Shaw
Affiliation:
Keywords: Boranophosphates, Natural Phosphodiesters, enzymatic polymerization, oligodeoxy nucleoside phosphorothioates, methylphosphonates, phosphoramidates, phosphoro fluoridates, F-ODN, Antisense Agents, Phosphodiester Analogs
Abstract: In boranophosphate-oligodeoxynucleosides (BH 3- -ODN) a borane group replaces one of the two non-bridging oxygen atoms in the phosphodiester backbone of the naturally occurring congener. The chemical and biophysical properties of BH 3 - -ODN are reviewed, as are their interactions with enzymes such as DNA polymerases, exo- and endonucleases, and ribonuclease H. Three approaches to synthesis of BH 3- -ODN are described in solution, on solid supports, and by template directed enzymatic polymerization of appropriately modified nucleoside triphosphates. Comparisons are made to other members of the family of phosphorus modified nucleic acids, the phosphorothioates and methylphosphonates. The potential applications of boranophosphate modified compounds to antisense therapeutics and DNA sequencing are discussed.
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Cite this article as:
Summers S. Jack and Shaw Ramsay Barbara, Boranophosphates as Mimics of Natural Phosphodiesters in DNA, Current Medicinal Chemistry 2001; 8 (10) . https://dx.doi.org/10.2174/0929867013372409
DOI https://dx.doi.org/10.2174/0929867013372409 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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