Abstract
Efficient development of stable formulations of protein pharmaceuticals requires an intimate knowledge of the protein and its chemical and physical properties. In particular, understanding the mechanisms by which a protein could degrade is critical for designing and testing formulations. This review describes the major pathways by which proteins can degrade, including denaturation, aggregation, oxidation, and interfacial damage. The methods to detect the degradation are covered, along with generalized strategies to retard or prevent each type of decomposition. Without an appreciation of the current best practices for devising stable formulations, the formulation process will be neither efficient nor optimal.
Keywords: Protein, Hydrophobic Interactions, Fluorescence Spectroscopy
Current Pharmaceutical Biotechnology
Title: The Stability Factor: Importance in Formulation Development
Volume: 3 Issue: 4
Author(s): Rajesh Krishnamurthy and Mark C. Manning
Affiliation:
Keywords: Protein, Hydrophobic Interactions, Fluorescence Spectroscopy
Abstract: Efficient development of stable formulations of protein pharmaceuticals requires an intimate knowledge of the protein and its chemical and physical properties. In particular, understanding the mechanisms by which a protein could degrade is critical for designing and testing formulations. This review describes the major pathways by which proteins can degrade, including denaturation, aggregation, oxidation, and interfacial damage. The methods to detect the degradation are covered, along with generalized strategies to retard or prevent each type of decomposition. Without an appreciation of the current best practices for devising stable formulations, the formulation process will be neither efficient nor optimal.
Export Options
About this article
Cite this article as:
Krishnamurthy Rajesh and Manning C. Mark, The Stability Factor: Importance in Formulation Development, Current Pharmaceutical Biotechnology 2002; 3 (4) . https://dx.doi.org/10.2174/1389201023378229
DOI https://dx.doi.org/10.2174/1389201023378229 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Mouse Models of Multiple Sclerosis: Lost in Translation?
Current Pharmaceutical Design Biologic Therapies in Primary Sjögren's Syndrome
Current Pharmaceutical Biotechnology Role of Natural Product in Modulation of Drug Transporters and New Delhi Metallo-β Lactamases
Current Topics in Medicinal Chemistry The Role of Chemokines in Alzheimer's Disease
Endocrine, Metabolic & Immune Disorders - Drug Targets Vitamin D Analogs in Cutaneous Malignancies
Current Pharmaceutical Design Protection from Radiation-induced Damage in Rat’s Ileum and Colon by Combined Regimens of Melatonin and Metformin: A Histopathological Study
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Type 2 Diabetes Mellitus and Invokana: An FDA Approved Drug
Current Diabetes Reviews Effects of Elicitors, Viticultural Factors, and Enological Practices on Resveratrol and Stilbenes in Grapevine and Wine
Mini-Reviews in Medicinal Chemistry <i>In Vivo</i> Anti-Tumor Effects of Flavokawain A in 4T1 Breast Cancer Cell-Challenged Mice
Anti-Cancer Agents in Medicinal Chemistry The Antidiabetic Potential of Quercetin: Underlying Mechanisms
Current Medicinal Chemistry Pharmacological Intervention of Cyclooxygenase-2 and 5-Lipoxygenase Pathways. Impact on Inflammation and Cancer
Current Pharmaceutical Design MicroRNAs and Cancer Therapy – From Bystanders to Major Players
Current Medicinal Chemistry Dissecting the Mechanisms of Thrombogenesis and Atherosclerosis for Neurodegenerative Disorders
Current Neurovascular Research P2RY12 Increased Neuroinflammation to Accelerate Depression-like Behaviors by the NLPR3 Inflammasome
Current Neurovascular Research Plastid Molecular Pharming II. Production of Biopharmaceuticals by Plastid Transformation
Mini-Reviews in Medicinal Chemistry Pharmacogenomics of Cardiovascular Complications in Diabetes and Obesity
Recent Patents on Biotechnology Preface [HotTopic: Novel Therapeutic Perspectives: The Targeted Inhibition of Genes and Proteins (Executive Editor: Felix Hoppe-Seyler)]
Current Molecular Medicine A Novel Class of Emerging Anticancer Compounds: Oxyprenylated Secondary Metabolites from Plants and Fungi
Current Medicinal Chemistry Review of the Currently Available Systems to Obtain Platelet Related Products to Treat Equine Musculoskeletal Injuries
Recent Patents on Regenerative Medicine Beta Caryophyllene and Caryophyllene Oxide, Isolated from Aegle Marmelos, as the Potent Anti-inflammatory Agents against Lymphoma and Neuroblastoma Cells
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry