Abstract
The epidermis is a model particularly well suited to the study of cell proliferation and differentiation, and of alterations of these processes such as carcinogenesis. Compartmentalization exists in this tissue, with the proliferative, less differentiated cells confined to the basal layer and the terminally differentiating, non-proliferative cells moving upwards to the surface through distinct layers. Different genes are expressed throughout this process in a stage-of-differentiationspecific manner, and their promoters have been very useful in directing precise gene expression in transgenic mice. Other attractive characteristics of the epidermis include its external localization, which facilitates manipulation and observation, the possibility of obtaining primary keratinocytes that can be easily cultured and manipulated in vitro, and the existence of well-established protocols for chemical and UV carcinogenesis. The latter are invaluable tools for assessing the in vivo functions of the genes targeted in transgenic mice. These characteristics have made the epidermis a widely used model system in recent years for the study of molecular mechanisms of carcinogenesis. A wealth of transgenic mice generated using epidermal-specific promoters, as well as knockout animals, have been used to examine the role of genes involved in processes such as cell cycle control, cell signaling, cell growth and differentiation, and angiogenesis in tumor and metastasis growth. Cre / loxP technology will allow a new generation of mice that allows the study of cancer genetics in a cell type-and time-controlled manner, more closely resembling the conditions found in the development of neoplasms.
Keywords: skin carcinogenesis, uv carcinogenesis, cre/loxp
Current Genomics
Title: Understanding Mouse Skin Carcinogenesis through Transgenic Approaches
Volume: 3 Issue: 4
Author(s): Fernando Larcher, Angel Ramirez, M. Llanos Casanova, Manuel Navarro, Jesus M. Paramio, Paloma Perez, Angustias Page, Mirentxu Santos and Jose L. Jorcano
Affiliation:
Keywords: skin carcinogenesis, uv carcinogenesis, cre/loxp
Abstract: The epidermis is a model particularly well suited to the study of cell proliferation and differentiation, and of alterations of these processes such as carcinogenesis. Compartmentalization exists in this tissue, with the proliferative, less differentiated cells confined to the basal layer and the terminally differentiating, non-proliferative cells moving upwards to the surface through distinct layers. Different genes are expressed throughout this process in a stage-of-differentiationspecific manner, and their promoters have been very useful in directing precise gene expression in transgenic mice. Other attractive characteristics of the epidermis include its external localization, which facilitates manipulation and observation, the possibility of obtaining primary keratinocytes that can be easily cultured and manipulated in vitro, and the existence of well-established protocols for chemical and UV carcinogenesis. The latter are invaluable tools for assessing the in vivo functions of the genes targeted in transgenic mice. These characteristics have made the epidermis a widely used model system in recent years for the study of molecular mechanisms of carcinogenesis. A wealth of transgenic mice generated using epidermal-specific promoters, as well as knockout animals, have been used to examine the role of genes involved in processes such as cell cycle control, cell signaling, cell growth and differentiation, and angiogenesis in tumor and metastasis growth. Cre / loxP technology will allow a new generation of mice that allows the study of cancer genetics in a cell type-and time-controlled manner, more closely resembling the conditions found in the development of neoplasms.
Export Options
About this article
Cite this article as:
Larcher Fernando, Ramirez Angel, Casanova Llanos M., Navarro Manuel, Paramio M. Jesus, Perez Paloma, Page Angustias, Santos Mirentxu and Jorcano L. Jose, Understanding Mouse Skin Carcinogenesis through Transgenic Approaches, Current Genomics 2002; 3 (4) . https://dx.doi.org/10.2174/1389202023350345
DOI https://dx.doi.org/10.2174/1389202023350345 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced AI Techniques in Big Genomic Data Analysis
The thematic issue on "Advanced AI Techniques in Big Genomic Data Analysis" aims to explore the cutting-edge methodologies and applications of artificial intelligence (AI) in the realm of genomic research, where vast amounts of data pose both challenges and opportunities. This issue will cover a broad spectrum of AI-driven strategies, ...read more
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Keeping A Breast of Recent Developments in Cancer Metabolism
Current Drug Targets MiRNA, a New Treatment Strategy for Pulmonary Fibrosis
Current Drug Targets Current Developments Using Emerging Transdermal Technologies In Physical Enhancement Methods.
Current Drug Delivery Factors Controlling Chromatin Organization and Nucleosome Positioning for Establishment and Maintenance of HIV Latency
Current HIV Research Soluble Adhesion Molecules in the Pathogenesis of Rheumatoid Arthritis
Current Pharmaceutical Design Chemokines in the Pathogenesis and as Therapeutical Markers and Targets of HCV Chronic Infection and HCV Extrahepatic Manifestations
Current Drug Targets Unique Structural Motif Supports Mannosylphospho Dolichol Synthase: An Important Angiogenesis Regulator
Current Drug Targets Cancer Prevention with Promising Natural Products: Mechanisms of Action and Molecular Targets
Anti-Cancer Agents in Medicinal Chemistry Differential Splicing, Disease and Drug Targets
Infectious Disorders - Drug Targets Regulation and Function of Antimicrobial Peptides in Immunity and Diseases of the Lung
Protein & Peptide Letters Progress Towards Clinically Useful Aldosterone Synthase Inhibitors
Current Topics in Medicinal Chemistry Probiotics as a Tool to Biosynthesize Metallic Nanoparticles: Research Reports and Patents Survey
Recent Patents on Drug Delivery & Formulation Collagen-Binding Integrins as Pharmaceutical Targets
Current Pharmaceutical Design Characterization of the Prostaglandin E2 Pathway in a Rat Model of Esophageal Adenocarcinoma
Current Cancer Drug Targets Emerging Roles for Modulation of microRNA Signatures in Cancer Chemoprevention
Current Cancer Drug Targets The Effect of Psychological Stress and Social Isolation on Neuroimmunoendocrine Communication
Current Pharmaceutical Design Diagnosis and Treatment of Paraneoplastic Neurological Syndromes
Current Clinical Pharmacology Thrombospondins as Anti-Angiogenic Therapeutic Agents
Current Pharmaceutical Design The Killing of Human Neuroblastoma Cells by the Small Molecule JQ1 Occurs in a p53-Dependent Manner
Anti-Cancer Agents in Medicinal Chemistry Signal Transduction via Cannabinoid Receptors
CNS & Neurological Disorders - Drug Targets