Abstract
Reverse genetics in Caenorhabditis elegans has had an enormous boost in the last decade. From the mere ability to inactivate genes and make transgenic animals in the late 1980s or early 1990s, this field has evolved to genome wide approaches that aim to inactivate all C. elegans genes and determine all expression patterns. Since this luxury position has not yet been attained, we still have to go through these experiments ourselves. Two different approaches exist to inactivate genes in C. elegans. One approach uses PCR to select for deletions in the gene of interest. Deletions can be generated using transposons or chemical mutagenesis. An alternative, or rather complementary, technique inactivates genes through RNA interference (RNAi). In this review I will describe these techniques and discuss their advantages and disadvantages. Finally, I will give an update on the current status of the genomic knockout projects.
Keywords: Gene inactivation, Caenorhabditis elegans, Rna interference(rnai), Transposon mutagenesis, Chemical mutagenesis, kutagenesis, Functional genomica
Current Genomics
Title: Gene Inactivation in Caenorhabditis elegans
Volume: 3 Issue: 2
Author(s): Gert Jansen
Affiliation:
Keywords: Gene inactivation, Caenorhabditis elegans, Rna interference(rnai), Transposon mutagenesis, Chemical mutagenesis, kutagenesis, Functional genomica
Abstract: Reverse genetics in Caenorhabditis elegans has had an enormous boost in the last decade. From the mere ability to inactivate genes and make transgenic animals in the late 1980s or early 1990s, this field has evolved to genome wide approaches that aim to inactivate all C. elegans genes and determine all expression patterns. Since this luxury position has not yet been attained, we still have to go through these experiments ourselves. Two different approaches exist to inactivate genes in C. elegans. One approach uses PCR to select for deletions in the gene of interest. Deletions can be generated using transposons or chemical mutagenesis. An alternative, or rather complementary, technique inactivates genes through RNA interference (RNAi). In this review I will describe these techniques and discuss their advantages and disadvantages. Finally, I will give an update on the current status of the genomic knockout projects.
Export Options
About this article
Cite this article as:
Jansen Gert, Gene Inactivation in Caenorhabditis elegans, Current Genomics 2002; 3 (2) . https://dx.doi.org/10.2174/1389202024605170
DOI https://dx.doi.org/10.2174/1389202024605170 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Current Genomics in Cardiovascular Research
Cardiovascular diseases are the main cause of death in the world, in recent years we have had important advances in the interaction between cardiovascular disease and genomics. In this Research Topic, we intend for researchers to present their results with a focus on basic, translational and clinical investigations associated with ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements