Abstract
Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.
Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases
Combinatorial Chemistry & High Throughput Screening
Title: Fluorescence Polarization Assays in Signal Transduction Discovery
Volume: 6 Issue: 3
Author(s): J. Richard Sportsman, Janet Daijo and Elizabeth A. Gaudet
Affiliation:
Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases
Abstract: Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.
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Cite this article as:
Sportsman Richard J., Daijo Janet and Gaudet A. Elizabeth, Fluorescence Polarization Assays in Signal Transduction Discovery, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298374
DOI https://dx.doi.org/10.2174/138620703106298374 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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