Abstract
Protein microarray technology allows the simultaneous determination of a large variety of parameters from a minute amount of sample within a single experiment. Assay systems based on this technology are currently applied for the identification, quantitation and functional analysis of proteins. Protein microarray technology is of major interest for proteomic research in basic and applied biology as well as for diagnostic applications. Miniaturized and parallelized assay systems have reached adequate sensitivity and hence have the potential to replace singleplex analysis systems. However, robustness and automation needs to be demonstrated before this technology will finally prove suitable for high-throughput applications. Miniaturized and parallelized sandwich immunoassays are the most advanced assays formats among the different protein microarray applications. Multiplexed sandwich immunoassays can be used for the identification of biomarkers and the validation of potential target molecules. In this review an overview will be given on the current stage of protein microarray technology with a special focus on miniaturized multiplexed sandwich immunoassays.
Keywords: Protein Microarrays, proteomic, immunoassays
Combinatorial Chemistry & High Throughput Screening
Title: Protein Microarrays and Multiplexed Sandwich Immunoassays: What Beats the Beads?
Volume: 7 Issue: 3
Author(s): Markus F. Templin, Dieter Stoll, Jutta Bachmann and Thomas O. Joos
Affiliation:
Keywords: Protein Microarrays, proteomic, immunoassays
Abstract: Protein microarray technology allows the simultaneous determination of a large variety of parameters from a minute amount of sample within a single experiment. Assay systems based on this technology are currently applied for the identification, quantitation and functional analysis of proteins. Protein microarray technology is of major interest for proteomic research in basic and applied biology as well as for diagnostic applications. Miniaturized and parallelized assay systems have reached adequate sensitivity and hence have the potential to replace singleplex analysis systems. However, robustness and automation needs to be demonstrated before this technology will finally prove suitable for high-throughput applications. Miniaturized and parallelized sandwich immunoassays are the most advanced assays formats among the different protein microarray applications. Multiplexed sandwich immunoassays can be used for the identification of biomarkers and the validation of potential target molecules. In this review an overview will be given on the current stage of protein microarray technology with a special focus on miniaturized multiplexed sandwich immunoassays.
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Cite this article as:
Templin F. Markus, Stoll Dieter, Bachmann Jutta and Joos O. Thomas, Protein Microarrays and Multiplexed Sandwich Immunoassays: What Beats the Beads?, Combinatorial Chemistry & High Throughput Screening 2004; 7 (3) . https://dx.doi.org/10.2174/1386207043328814
DOI https://dx.doi.org/10.2174/1386207043328814 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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