Abstract
Mycobacteria, especially M. tuberculosis, have remained a worldwide dominant cause for human morbidity (∼10 million annual cases) and mortality (3 million deaths annually) since ancient times. An estimated one-third of living humans are latently infected with M. tuberculosis. Despite this, there have been no new drugs specifically developed against mycobacteria since the 1960s. Because of a slow growth rate, biosafety concerns, and other issues, drug discovery by in vitro screening of natural and synthetic compounds has been limited in the past. However, a number of new techniques and technologies for whole cell antimycobacterial screening have been developed recently. This review examines and compares these methods, discusses common issues in screening with mycobacteria, and considers potential future developments in the field.
Keywords: Mycobacterium, drug discovery, resazurin, alamar blue, nitrate reductase, luciferase, green fluorescent protein
Current Bioactive Compounds
Title: Recent Advances in Methodologies for the Discovery of Antimycobacterial Drugs
Volume: 3 Issue: 3
Author(s): Todd P. Primm and Scott G. Franzblau
Affiliation:
Keywords: Mycobacterium, drug discovery, resazurin, alamar blue, nitrate reductase, luciferase, green fluorescent protein
Abstract: Mycobacteria, especially M. tuberculosis, have remained a worldwide dominant cause for human morbidity (∼10 million annual cases) and mortality (3 million deaths annually) since ancient times. An estimated one-third of living humans are latently infected with M. tuberculosis. Despite this, there have been no new drugs specifically developed against mycobacteria since the 1960s. Because of a slow growth rate, biosafety concerns, and other issues, drug discovery by in vitro screening of natural and synthetic compounds has been limited in the past. However, a number of new techniques and technologies for whole cell antimycobacterial screening have been developed recently. This review examines and compares these methods, discusses common issues in screening with mycobacteria, and considers potential future developments in the field.
Export Options
About this article
Cite this article as:
Primm P. Todd and Franzblau G. Scott, Recent Advances in Methodologies for the Discovery of Antimycobacterial Drugs, Current Bioactive Compounds 2007; 3 (3) . https://dx.doi.org/10.2174/157340707781695550
DOI https://dx.doi.org/10.2174/157340707781695550 |
Print ISSN 1573-4072 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6646 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Discovery of Potential Dual Inhibitors Against Lipases Rv0183 and Rv3802c for Tuberculosis Therapeutics
Letters in Drug Design & Discovery Microwave-assisted Single Step Cinnamic Acid Derivatization and Evaluation for Cytotoxic Potential
Current Pharmaceutical Biotechnology The Mechanism of Adjuvanticity of Aluminium-Containing Formulas
Current Pharmaceutical Design Editorial [ The Reversal of Drug Resistance from Bacteria to Cancer Cells Part - I Guest Editor: Joseph Molnar ]
Current Drug Targets Structure and Function of MPN (Mpr1/Pad1 N-terminal) Domain- Containing Proteins
Current Protein & Peptide Science Inflammatory Syndrome in Chronic Kidney Disease: Pathogenesis and Influence on Outcomes
Inflammation & Allergy - Drug Targets (Discontinued) Pharmacogenomics – Implications in the Development of HIV-Associated Brain Disease
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Shikimate Kinase, a Protein Target for Drug Design
Current Medicinal Chemistry Recent Patents on Immunoregulatory DNA Vaccines for Autoimmune Diseases and Allograft Rejection
Recent Patents on DNA & Gene Sequences Editorial (Thematic Issue: Techniques and Application in Metabolomics: From Metabolite Profiling to Spatial Distribution)
Current Metabolomics Potential Use of Protease Inhibitors as Vaginal and Colorectal Microbicides
Current HIV Research Patent Selections:
Recent Patents on Anti-Infective Drug Discovery Burden of Multidrug Resistant Mycobacterium tuberculosis Among New Cases in Al-Madinah Al-Monawarah, Saudi Arabia
Infectious Disorders - Drug Targets New Insights into Lipidic Secondary Metabolites in Mycobacteria
Current Chemical Biology Novel Azole-Functionalited Flouroquinolone Hybrids: Design, Conventional and Microwave Irradiated Synthesis, Evaluation as Antibacterial and Antioxidant Agents
Letters in Drug Design & Discovery Recent Patents of Gene Sequences Relative to DNA Polymerases
Recent Patents on DNA & Gene Sequences Trace and Minor Element Concentrations in Aqueous Extract of the Herbal Medicinal Plants Tamarindus indica and Hibiscus sabdiffora
Current Nutrition & Food Science Molecular Docking Algorithms
Current Drug Targets Amyloid A Amyloidosis Secondary to Rheumatoid Arthritis: An Uncommon Yet Important Complication
Current Rheumatology Reviews Slow-Onset Inhibition of 2-trans-Enoyl-ACP (CoA) Reductase from Mycobacterium tuberculosis by an Inorganic Complex
Current Pharmaceutical Design