Chloroplast Protein Import: Reverse Genetic Approaches

Title: Chloroplast Protein Import: Reverse Genetic Approaches

Volume: 7 Issue: 4

Author(s): Mislav Oreb, Katharina Reger and Enrico Schleiff

Affiliation:

Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana

Abstract: Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.

Cite this article as:

Oreb Mislav, Reger Katharina and Schleiff Enrico, Chloroplast Protein Import: Reverse Genetic Approaches, Current Genomics 2006; 7 (4) . https://dx.doi.org/10.2174/138920206778427004