Abstract
Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Current Genomics
Title: Chloroplast Protein Import: Reverse Genetic Approaches
Volume: 7 Issue: 4
Author(s): Mislav Oreb, Katharina Reger and Enrico Schleiff
Affiliation:
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Abstract: Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
Export Options
About this article
Cite this article as:
Oreb Mislav, Reger Katharina and Schleiff Enrico, Chloroplast Protein Import: Reverse Genetic Approaches, Current Genomics 2006; 7 (4) . https://dx.doi.org/10.2174/138920206778427004
DOI https://dx.doi.org/10.2174/138920206778427004 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Current Genomics in Cardiovascular Research
Cardiovascular diseases are the main cause of death in the world, in recent years we have had important advances in the interaction between cardiovascular disease and genomics. In this Research Topic, we intend for researchers to present their results with a focus on basic, translational and clinical investigations associated with ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Vitamin D Analogs as Anti-Carcinogenic Agents
Anti-Cancer Agents in Medicinal Chemistry Structure, Function, and Pathogenesis of SHP2 in Developmental Disorders and Tumorigenesis
Current Cancer Drug Targets Membrane Transporters as Determinants of the Pharmacology of Platinum Anticancer Drugs
Current Cancer Drug Targets The Cardiotoxicity of Local Anesthetics: The Place of Ropivacaine
Current Topics in Medicinal Chemistry Targeting Adhesion Molecules in Cardiovascular Disorders
Cardiovascular & Hematological Disorders-Drug Targets Neonatal Ultrasound in Transport
Current Pediatric Reviews Nutritional Control, Gene Regulation, and Transformation of Vascular Smooth Muscle Cells in Atherosclerosis
Cardiovascular & Hematological Disorders-Drug Targets Promises of Apoptosis-Inducing Peptides in Cancer Therapeutics
Current Pharmaceutical Biotechnology The Potential of Secondary Metabolites from Plants as Drugs or Leads Against Protozoan Neglected Diseases - Part II
Current Medicinal Chemistry Natural Product-Derived Small Molecule Activators of Hypoxia-Inducible Factor-1 (HIF-1)
Current Pharmaceutical Design Perspectives on Medicinal Properties of Benzoquinone Compounds
Mini-Reviews in Medicinal Chemistry Anesthesia and Postoperative Cognitive Dysfunction (POCD)
Central Nervous System Agents in Medicinal Chemistry Ca<sup>2+</sup> Signalling in Endothelial Progenitor Cells: A Novel Means to Improve Cell-Based Therapy and Impair Tumour Vascularisation
Current Vascular Pharmacology Medicinal Plants and Cancer Chemoprevention
Current Drug Metabolism Insights on the Neuromodulatory Propensity of Selaginella (Sanjeevani) and its Potential Pharmacological Applications
CNS & Neurological Disorders - Drug Targets LFA-1 as a Key Regulator of Immune Function: Approaches toward the Development of LFA-1-Based Therapeutics
Current Pharmaceutical Design Role of Histone Deacetylases (HDACs) in Epilepsy and Epileptogenesis
Current Pharmaceutical Design Vascular Disease and Risk Factors in Chronic Kidney Disease
Vascular Disease Prevention (Discontinued) Uptake and Metabolism of the Short-Chain Fatty Acid Butyrate, a Critical Review of the Literature
Current Drug Metabolism Therapeutical Potential of CB<sub>2</sub> Receptors in Immune-Related Diseases
Current Molecular Pharmacology