Abstract
The rapid development of proteomics has been made possible primarily by the progress in analytical and preparative separation methods. Biological systems are so complex that the separation procedures employed must be highly efficient as well as highly selective. The latter requirement is best met by affinity separations based on molecular recognition. The present review critically discusses the properties of the affinity sorbents employed in proteomics, namely, agarose gels, dextrans, modified methacrylate and acrylamide polymers, porous and nonporous silica, porous glass beads, monoliths, affinity membranes and magnetic beads. The physico-chemical properties of these materials, their preparation, application, approaches to their modification and their relative advantages and drawbacks are discussed.
Keywords: Affinity chromatography, proteins, supports, proteomic applications
Current Proteomics
Title: Separation Media in Affinity Chromatography of Proteins - A Critical Review
Volume: 3 Issue: 1
Author(s): Tereza Varilova, Milan Madera, Vera Pacakova and Karel Stulik
Affiliation:
Keywords: Affinity chromatography, proteins, supports, proteomic applications
Abstract: The rapid development of proteomics has been made possible primarily by the progress in analytical and preparative separation methods. Biological systems are so complex that the separation procedures employed must be highly efficient as well as highly selective. The latter requirement is best met by affinity separations based on molecular recognition. The present review critically discusses the properties of the affinity sorbents employed in proteomics, namely, agarose gels, dextrans, modified methacrylate and acrylamide polymers, porous and nonporous silica, porous glass beads, monoliths, affinity membranes and magnetic beads. The physico-chemical properties of these materials, their preparation, application, approaches to their modification and their relative advantages and drawbacks are discussed.
Export Options
About this article
Cite this article as:
Varilova Tereza, Madera Milan, Pacakova Vera and Stulik Karel, Separation Media in Affinity Chromatography of Proteins - A Critical Review, Current Proteomics 2006; 3 (1) . https://dx.doi.org/10.2174/157016406777145685
DOI https://dx.doi.org/10.2174/157016406777145685 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Mechanical Imaging - a Technology for 3-D Visualization and Characterization of Soft Tissue Abnormalities: A Review
Current Medical Imaging DHODH Hot Spots: An Underexplored Source to Guide Drug Development Efforts
Current Topics in Medicinal Chemistry Label-Free Cell Phenotypic Drug Discovery
Combinatorial Chemistry & High Throughput Screening Breast Cancer Resistance Protein: A Potential Therapeutic Target for Cancer
Current Drug Targets Therapeutic and Protective Potential of Mesenchymal Stem Cells, Pharmaceutical Agents and Current Vaccines Against COVID-19
Current Stem Cell Research & Therapy Exosomes: A Role for Naturally Occurring Nanovesicles in Cancer Growth, Diagnosis and Treatment
Current Gene Therapy Synthesis and Antitumor Activity of Novel 4-Chloro-3-Arylmaleimide Derivatives
Letters in Drug Design & Discovery Application of Solvent Influenced Fluorescence-quenching and Enhancement to Develop a Highly Sensitive HPLC Methodology for Analysis of Resveratrol-PEG Conjugates
Current Pharmaceutical Analysis Protein Bioinformatics Applied to Virology
Current Protein & Peptide Science From Na+/K+-ATPase and Cardiac Glycosides to Cytotoxicity and Cancer Treatment
Anti-Cancer Agents in Medicinal Chemistry Investigation of Antioxidant Activity and Analysis of Phenolic Compounds of some Asteraceae Plants by HPLC: A Comparison Between Methanol and Ethanol Extracts
Current Nutraceuticals Magnetic Nanoparticles for Cancer Therapy
Current Nanoscience A Search for Inhibitors of S100B, a Member of the S100 Family of Calcium-Binding Proteins
Mini-Reviews in Medicinal Chemistry Bladder Cancer: Molecular Determinants of Personalized Therapy
Current Drug Targets Multiple Activities in Lantibiotics - Models for the Design of Novel Antibiotics?
Current Pharmaceutical Design Biomolecular Pathogenesis of Borderline Ovarian Tumors: Focusing Target Discovery Through Proteogenomics
Current Cancer Drug Targets Computer Aided Drug Design for Multi-Target Drug Design: SAR /QSAR, Molecular Docking and Pharmacophore Methods
Current Drug Targets Synthesis and Evaluation of a New Series of Arylidene Indanones as Potential Anticancer Agents
Anti-Cancer Agents in Medicinal Chemistry HIV-1 Matrix Protein p17 and its Receptors
Current Drug Targets A Comprehensive Proteomic Study of the Skin Secretions of the Frog Lithobates spectabilis
Protein & Peptide Letters