Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells

Title: Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells

Volume: 13 Issue: 4

Author(s): Judith C. Bandsmer, Tessa N. Campbell, Ian Cheyne and Francis Y.M. Choy

Affiliation:

Keywords: TAT, α-N-acetylglucosaminidase, mucopolysaccharidosis, Sanfilippo, protein expression

Abstract: We examined the production and secretion of fusion constructs containing α-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/α-Nacetylglucosaminidase chimerae in enzyme replacement therapy.

Cite this article as:

Bandsmer C. Judith, Campbell N. Tessa, Cheyne Ian and Choy Y.M. Francis, Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974456