Abstract
Chemico-enzymatic synthesis and cloning in Esherichia coli of an artificial gene coding human glucagon was performed. Recombinant plasmid containing hybrid glucagons gene and intein Ssp dnaB from Synechocestis sp. was designed. Expression of the obtained hybrid gene in E. coli, properties of the formed hybrid protein, and conditions of its autocatalytic cleavage leading to glucagon formation were studied.
Keywords: Glucagon, mini-intein, autocatalitic cleavage, recombinant, polypeptide
Protein & Peptide Letters
Title: Production and Purification of Recombinant Human Glucagon Overexpressed as Intein Fusion Protein in Escherichia coli
Volume: 13 Issue: 4
Author(s): Roman S. Esipov, Vasily N. Stepanenko, Alexandr I. Gurevich, Larisa A. Chupova and Anatoly I. Miroshnikov
Affiliation:
Keywords: Glucagon, mini-intein, autocatalitic cleavage, recombinant, polypeptide
Abstract: Chemico-enzymatic synthesis and cloning in Esherichia coli of an artificial gene coding human glucagon was performed. Recombinant plasmid containing hybrid glucagons gene and intein Ssp dnaB from Synechocestis sp. was designed. Expression of the obtained hybrid gene in E. coli, properties of the formed hybrid protein, and conditions of its autocatalytic cleavage leading to glucagon formation were studied.
Export Options
About this article
Cite this article as:
Esipov S. Roman, Stepanenko N. Vasily, Gurevich I. Alexandr, Chupova A. Larisa and Miroshnikov I. Anatoly, Production and Purification of Recombinant Human Glucagon Overexpressed as Intein Fusion Protein in Escherichia coli, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974320
DOI https://dx.doi.org/10.2174/092986606775974320 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
GnRH Agonists and Antagonists in Cancer Therapy
Current Medicinal Chemistry - Anti-Cancer Agents Importance and Potential of Dentists in Identifying Patients at High Risk of Diabetes
Current Diabetes Reviews Transdermal Delivery of Atenolol: Effect of Prodrugs and Iontophoresis
Current Drug Delivery Deciphering a Serum Peptide Activity Associated with Biologic Clock
Protein & Peptide Letters Subject Index To Volume 2
Current Respiratory Medicine Reviews The Corticotropin-Releasing Factor (CRF) Family of Neuropeptides in Inflammation: Potential Therapeutic Applications
Current Medicinal Chemistry Approaches for Administering Chemotherapy in the Intensive Care Unit
Current Drug Safety JNK Signalling: A Possible Target to Prevent Neurodegeneration
Current Pharmaceutical Design Cognitive Function and Quality of Life in Mild Thyroid Hormone Deficiency
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery The Use of Cyclodextrins Nanoparticles for Oral Delivery
Current Medicinal Chemistry Therapeutic Implications of Tocilizumab, A Humanized Anti-Interleukin-6 Receptor Antibody, for Various Immune-Mediated Diseases: An Update Review
Current Rheumatology Reviews Smoking and Eye Pathologies. A Systemic Review. Part II. Retina Diseases, Uveitis, Optic Neuropathies, Thyroid-Associated Orbitopathy
Current Pharmaceutical Design Inflammatory Risk Factors and Pathologies Associated with Alzheimers Disease
Current Alzheimer Research Current Advances in Delivery of Biotherapeutics Across the Blood-Brain Barrier
Current Drug Discovery Technologies COVID-19 Associated Rhino-Orbital-Cerebral Mucormycosis: A Proposed Classification and Treatment Strategies
Infectious Disorders - Drug Targets Neuro-psychopharmacogenetics and Neurological Antecedents of Posttraumatic Stress Disorder: Unlocking the Mysteries of Resilience and Vulnerability
Current Neuropharmacology CCR2 Antagonists
Current Topics in Medicinal Chemistry Transthyretin and the Systemic Inflammatory Response
Current Nutrition & Food Science Role of Renin-Angiotensin System in Vascular Endothelial Dysfunction of Pregnancy-Induced Hypertension
Current Hypertension Reviews The Dramatic Role of IFN Family in Aberrant Inflammatory Osteolysis
Current Gene Therapy