Abstract
There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.
Keywords: Crystallography, protein expression, purification, pTIg-BOS-Fc
Protein & Peptide Letters
Title: Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification
Volume: 13 Issue: 2
Author(s): Bryan W. Day, Fiona M. Smith, Ke Chen, Jennifer K. McCarron, Nirmitha I. Herath, Martin Lackmann and Andrew W. Boyd
Affiliation:
Keywords: Crystallography, protein expression, purification, pTIg-BOS-Fc
Abstract: There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.
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Cite this article as:
Day W. Bryan, Smith M. Fiona, Chen Ke, McCarron K. Jennifer, Herath I. Nirmitha, Lackmann Martin and Boyd W. Andrew, Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification, Protein & Peptide Letters 2006; 13 (2) . https://dx.doi.org/10.2174/092986606775101625
DOI https://dx.doi.org/10.2174/092986606775101625 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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