Abstract
The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The aberrant or accidental methylation of CpG islands in the promoter region has been observed for many cancer-related genes resulting in the silencing of their expression. Hence, it has become a focus of scientific attraction currently. Many of the early procedures developed gave only somewhat limited information about methylation patterns, for example, the overall levels of 5-methycytosine in the genome or the frequency of methylation of cytosines within certain restriction sites. However, in the last few years, there has been an explosion of interest in DNA methylation, and with it, many new and powerful techniques have been developed to facilitate methylation analysis, especially the advent of many high-throughput methods (e.g., restriction landmark genome scanning, differential methylation hybridization, and DNA microarray). In this paper, the key techniques currently available are reviewed and the advantages and disadvantages are discussed. This review will be valuable in selecting the best techniques to address particular questions concerning methylation status of genome DNA.
Keywords: DNA methylation, Epigenetic, Gene expression, Nonspecific methylation analysis, Specific methylation analysis
Current Analytical Chemistry
Title: A Profile of Current Methods for DNA Methylation Analysis
Volume: 2 Issue: 3
Author(s): Peng Hou, Meiju Ji, Zaozao Chen and Zuhong Lu
Affiliation:
Keywords: DNA methylation, Epigenetic, Gene expression, Nonspecific methylation analysis, Specific methylation analysis
Abstract: The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The aberrant or accidental methylation of CpG islands in the promoter region has been observed for many cancer-related genes resulting in the silencing of their expression. Hence, it has become a focus of scientific attraction currently. Many of the early procedures developed gave only somewhat limited information about methylation patterns, for example, the overall levels of 5-methycytosine in the genome or the frequency of methylation of cytosines within certain restriction sites. However, in the last few years, there has been an explosion of interest in DNA methylation, and with it, many new and powerful techniques have been developed to facilitate methylation analysis, especially the advent of many high-throughput methods (e.g., restriction landmark genome scanning, differential methylation hybridization, and DNA microarray). In this paper, the key techniques currently available are reviewed and the advantages and disadvantages are discussed. This review will be valuable in selecting the best techniques to address particular questions concerning methylation status of genome DNA.
Export Options
About this article
Cite this article as:
Hou Peng, Ji Meiju, Chen Zaozao and Lu Zuhong, A Profile of Current Methods for DNA Methylation Analysis, Current Analytical Chemistry 2006; 2 (3) . https://dx.doi.org/10.2174/157341106777698198
DOI https://dx.doi.org/10.2174/157341106777698198 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Proteomics Analysis of Liver Tissue of Labeo rohita
Current Proteomics Assessment of the Health Effects of Chemicals in Humans: I. QSAR Estimation of the Maximum Recommended Therapeutic Dose (MRTD) and No Effect Level (NOEL) of Organic Chemicals Based on Clinical Trial Data1
Current Drug Discovery Technologies Syntheses and In-Vitro Evaluation of Novel Adamantane Based γ-Secretase Inhibitors
Current Medicinal Chemistry Application of Charge Transfer Reactions for the Quantitative Spectrophotometric Determination of Cyclophosphamide in Pure and Pharmaceutical Formulation
Current Pharmaceutical Analysis Determination of Dysregulated miRNA Expression Levels by qRT-PCR after the Application of Usnic Acid to Breast Cancer
Anti-Cancer Agents in Medicinal Chemistry Surface Interactions: Functionalization of Graphene Oxide and Wetting of Graphene Oxide and Graphene
Current Organic Chemistry Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis
Protein & Peptide Letters Using Pseudo Amino Acid Composition to Predict Protease Families by Incorporating a Series of Protein Biological Features
Protein & Peptide Letters Antioxidant Properties of Kalanchoe blossfeldiana – A Focus on Erythrocyte Membrane Stability and Cytoprotection
Current Traditional Medicine Role of Moving Average Analysis for Development of Multi-Target (Q)SAR Models
Mini-Reviews in Medicinal Chemistry Viruses in Semen and Male Genital Tissues - Consequences for the Reproductive System and Therapeutic Perspectives
Current Pharmaceutical Design Advances in Computational Structure-Based Drug Design and Application in Drug Discovery
Current Topics in Medicinal Chemistry A Review on Medical Image Registration as an Optimization Problem
Current Medical Imaging Functional Nucleic Acids in High Throughput Screening and Drug Discovery
Combinatorial Chemistry & High Throughput Screening Sigma-2 Receptor Ligands: Neurobiological Effects
Current Medicinal Chemistry Legumes: A Healthy and Ecological Source of Flavonoids
Current Nutrition & Food Science Resveratrol Promotes HIV-1 Tat Accumulation <i>via</i> AKT/FOXO1 Signaling Axis and Potentiates Vorinostat to Antagonize HIV-1 Latency
Current HIV Research Optimization of Aqueous Extraction of the Australian Maroon Bush (<i>Scaevola Spinescens</i> R. Br.) to Maximize Bioactive Compound and Antioxidant Yield
Current Nutraceuticals Synthesis of Steroidal Mimics of Estrogens Using Wittig and McMurry Reactions
Letters in Organic Chemistry Therapeutic Potential of Plant Extracts and Phytochemicals Against Brain Ischemia-Reperfusion Injury: A Review
The Natural Products Journal