Abstract
The discovery and utility of new methods based on different materials and chemical derivatisations for protein profiling of complex analytes is an ongoing field. This study includes the development, optimisation, validation and the application of a method based on the combined characteristics of physisorption and chemisorption of nano crystalline diamond (NCD) surfaces for serum profiling. Due to the expanded nano-structure, the numbers of potential binding sites are dramatically increased. For immobilised metal ion affinity chromatography (IMAC), diamond coated surfaces were derivatised with glycidyl methacrylate (GMA) under ultraviolet (UV) light at different wavelengths followed by further derivatisation with iminodiacetic acid (IDA) and loading with copper ions. This special kind of MALDI/TOF-MS, was termed as MELDI/TOF-MS (Material enhanced laser desorption ionization time of flight mass spectrometry), as it is based on various materials e.g. diamond, cellulose, silica and poly (glycidyl methacrylate/divinylbenzene). The diversity in the physical characteristics of these derivatised materials is responsible to get improved sensitivity, specificity, capacity and broad range of information. Human serum samples were assayed through MELDI/TOF-MS analysis to validate the capability, capacity, efficiency and reproducibility of the nano-structured diamond surfaces for protein profiling. Also the method was employed to search for the differences in human body fluids e.g. sera of different origin through matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI/TOF-MS).
Keywords: MELDI, Nano crystalline diamond, Functionalisation, Mass finger printing, Regeneration, XPS
Current Nanoscience
Title: Chemically Modified Nano Crystalline Diamond Layer as Material Enhanced Laser Desorption Ionisation (MELDI) Surface in Protein Profiling
Volume: 2 Issue: 1
Author(s): M. Najam-ul-Haq, M. Rainer, C. W. Huck, G. Stecher, I. Feuerstein, D. Steinmuller and G. K. Bonn
Affiliation:
Keywords: MELDI, Nano crystalline diamond, Functionalisation, Mass finger printing, Regeneration, XPS
Abstract: The discovery and utility of new methods based on different materials and chemical derivatisations for protein profiling of complex analytes is an ongoing field. This study includes the development, optimisation, validation and the application of a method based on the combined characteristics of physisorption and chemisorption of nano crystalline diamond (NCD) surfaces for serum profiling. Due to the expanded nano-structure, the numbers of potential binding sites are dramatically increased. For immobilised metal ion affinity chromatography (IMAC), diamond coated surfaces were derivatised with glycidyl methacrylate (GMA) under ultraviolet (UV) light at different wavelengths followed by further derivatisation with iminodiacetic acid (IDA) and loading with copper ions. This special kind of MALDI/TOF-MS, was termed as MELDI/TOF-MS (Material enhanced laser desorption ionization time of flight mass spectrometry), as it is based on various materials e.g. diamond, cellulose, silica and poly (glycidyl methacrylate/divinylbenzene). The diversity in the physical characteristics of these derivatised materials is responsible to get improved sensitivity, specificity, capacity and broad range of information. Human serum samples were assayed through MELDI/TOF-MS analysis to validate the capability, capacity, efficiency and reproducibility of the nano-structured diamond surfaces for protein profiling. Also the method was employed to search for the differences in human body fluids e.g. sera of different origin through matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI/TOF-MS).
Export Options
About this article
Cite this article as:
Najam-ul-Haq M., Rainer M., Huck W. C., Stecher G., Feuerstein I., Steinmuller D. and Bonn K. G., Chemically Modified Nano Crystalline Diamond Layer as Material Enhanced Laser Desorption Ionisation (MELDI) Surface in Protein Profiling, Current Nanoscience 2006; 2 (1) . https://dx.doi.org/10.2174/157341306775473836
DOI https://dx.doi.org/10.2174/157341306775473836 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Guaraná a Caffeine-Rich Food Increases Oxaliplatin Sensitivity of Colorectal HT-29 Cells by Apoptosis Pathway Modulation
Anti-Cancer Agents in Medicinal Chemistry Recent Development of Peptide Drugs and Advance on Theory and Methodology of Peptide Inhibitor Design
Medicinal Chemistry Daidzein and its Effects on Brain
Current Medicinal Chemistry Complex Interactions between Phytochemicals. The Multi-Target Therapeutic Concept of Phytotherapy
Current Drug Targets Imaging of Tumor Angiogenesis: Current Approaches and Future Prospects
Current Pharmaceutical Design The Role of NPY and Ghrelin in Anorexia Nervosa
Current Pharmaceutical Design Retinoic Acid Signaling in P19 Stem Cell Differentiation
Anti-Cancer Agents in Medicinal Chemistry Instructions from the Vascular System - Directing Neural Stem Cell Fate in Health and Disease
Current Medicinal Chemistry Meet Our Editorial Board Member:
Current Pharmaceutical Design Quantum Dots and Their Clinical Applications
Nanoscience & Nanotechnology-Asia In Vivo Transdermal Delivery of Leuprolide Using Microneedles and Iontophoresis
Current Pharmaceutical Biotechnology Prostaglandins Pathway as a Possible Biological Link Between Cancer and Major Depression
Current Angiogenesis (Discontinued) Gold Nanostructures as Photothermal Therapy Agent for Cancer
Anti-Cancer Agents in Medicinal Chemistry Dietary Agents for Prostate Cancer Chemoprevention: An Overview
Current Cancer Therapy Reviews Benzofuran Small Molecules as Potential Inhibitors of Human Protein Kinases. A Review
Current Pharmaceutical Design The Emerging Role of Endocrine Disruptors in Pathogenesis of Insulin Resistance: A Concept Implicating Nonalcoholic Fatty Liver Disease
Current Molecular Medicine Metal Based Frameworks for Drug Delivery Systems
Current Topics in Medicinal Chemistry The Ca2+-Activated K+ Channel of Intermediate Conductance:A Molecular Target for Novel Treatments?
Current Drug Targets Advancements within Modern Machine Learning Methodology: Impacts and Prospects in Biomarker Discovery
Current Medicinal Chemistry Harnessing the Capacity of Cell-Penetrating Peptides for Drug Delivery to the Central Nervous System
Current Pharmaceutical Biotechnology