Background: Extremophilic microorganisms from a wide variety of extreme natural environments
have been researched, and many biotechnological applications have been carried out, due to their capacity to
produce biomolecules resistant to extreme conditions, such as fibrinolytic proteases. The search for new fibrinolytic
enzymes is important in the development of new therapies against cardiovascular diseases.
Objective: This article aimed to evaluate the patents filed about protease with fibrinolytic activity produced by
extremophilic microorganisms whose use is aimed at the development of new drugs for the treatment of cardiovascular
Methods: The prospecting was carried out using data on deposits and patent concessions made available on the
technological bases: European Patent Office (EPO), United States Patent and Trademark Office (USPTO),
World Intellectual Property Organization (WIPO), Instituto Nacional de Propriedade Industrial – Brazil (INPI),
The LENS and Patent Inspiration. The International Patent Classification and subclasses and groups for each
document were also evaluated.
Results: Although 382 patents were selected using terms related to extreme environments, such as “thermophile”
and “acidophiles”, few were related to clinical use and were mainly performed using Bacillus subtilis
and Streptomyces megasporus strains. A highlight of nattokinase was produced by Bacillus subtilis GDN and
actinokinase by Streptomyces megasporus SD5.
Conclusion: The low number of patents on enzymes with this profile (extreme environments) revealed a little--
explored field, promising in the development of new microbial thrombolytic drugs, such as fibrinolytic enzymes
with less adverse effects.