Login Register Cart 0
Generic placeholder image

Current Molecular Medicine

Editor-in-Chief

ISSN (Print): 1566-5240
ISSN (Online): 1875-5666

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe Translate in Chinese
Research Article

Diet-induced Obesity Differentially Modulates Cardiac Inflammatory Status in the C57 and FVB Mouse Strains

Author(s): Fernanda Gaisler-Silva, Carolina Victoria Cruz Junho, Izabelle Fredo-da-Costa, Marcelo Augusto Christoffolete and Marcela Sorelli Carneiro-Ramos*

Volume 22, Issue 4, 2022

Published on: 06 July, 2021

Page: [365 - 373] Pages: 9

DOI: 10.2174/1566524021666210603163613

Abstract

Background: Cardiovascular diseases correspond to the highest risk of sudden death worldwide, and obesity is largely related to be an increased risk factor. There is a higher prevalence of arterial hypertension in obese individuals, including the presence of cardiac hypertrophy. It is already known the role of toll-like receptors [TLR], mainly 2 and 4 in heart cells, as fundamental to the process of cardiac hypertrophy. Obesity has been studied as an activator of damage-associated molecular patterns [DAMPs], which use the TLR signaling pathway to increase the nuclear factor of inflammation, NF-kB, increasing cytokine expression in heart tissue. It’s already known that FVB/N and C57BL/6 mouse strains have different behaviors in relation to metabolism, but the difference in cardiac tropism and innate immune system modulation are not clear.

Methods: The present study aimed to evaluate the contribution of innate immune factors to cardiac hypertrophy induced by an experimental model of obesity comparing two mouse strains: C57BL/6 and FVB/N. Both strains were submitted to a high-fat diet containing 23% protein, 35.5% carbohydrate, and 35.9% fat for 68 days. Hearts were collected, weighed, and submitted to RT-qPCR, and the serum was analyzed by Bioplex.

Results: We observed an increase in heart mass after 68 days in both strains. This was followed by an increase of α-actin only in C57BL/6 while ANF was increased in FVB/N. Gene expression of innate immune components and inflammatory cytokines were only increased in C57BL/6, but not in FVB/N.

Conclusion: Based on the results obtained, we verified that C57BL/6 mice had a more robust action of innate immune system then FVB/N.

Keywords: Cardiac hypertrophy, obesity, high-fat diet, immune response, TLR, C57BL/6, FVB/N.

« Previous Next »
[1]
Hernandes F, Valentini MP. Obesidade: Causas e consequências em crianças e adolescentes. Conexões (Campinas) 2010; 8(3): 47-63. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/conexoes/article/view/8637727
[http://dx.doi.org/10.20396/conex.v8i3.8637727]
[2]
Seravalle G, Grassi G. Obesity and hypertension. Pharmacol Res 2017; 122: 1-7. Available from: https://linkinghub. elsevier.com/retrieve/pii/S1043661817304620
[http://dx.doi.org/10.1016/j.phrs.2017.05.013] [PMID: 28532816]
[3]
Liu Y-J, Araujo S, Recker RR, Deng H-W. Molecular and genetic mechanisms of obesity: Implications for future management. Curr Mol Med 2003; 3(4): 325-40. Available from: http://www.eurekaselect.com/openurl/content.php?genre=article&issn=1566-5240&volume=3&issue=4&spage=325
[http://dx.doi.org/10.2174/1566524033479735] [PMID: 12776988]
[4]
Wasim M, Awan FR, Najam SS, Khan AR, Khan HN. Role of leptin deficiency, inefficiency, and leptin receptors in obesity. Biochem Genet 2016; 54(5): 565-72.
[http://dx.doi.org/10.1007/s10528-016-9751-z] [PMID: 27313173]
[5]
Romero CEM, Zanesco A. O papel dos hormônios leptina e grelina na gênese da obesidade. Rev Nutr 2006; 19(1): 85-91. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732006000100009&lng=pt&tlng=pt
[http://dx.doi.org/10.1590/S1415-52732006000100009]
[6]
Suresh Y, Das UN. Leptin-the fat controller. J Assoc Physicians India 1998; 46(6): 538-44. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11273255
[PMID: 11273255]
[7]
Abella V, Scotece M, Conde J, et al. Leptin in the interplay of inflammation, metabolism and immune system disorders. Nat Rev Rheumatol 2017; 13(2): 100-9. Available from: http://www.nature.com/articles/nrrheum.2016.209
[http://dx.doi.org/10.1038/nrrheum.2016.209] [PMID: 28053336]
[8]
Lu YC, Yeh WC, Ohashi PS. LPS/TLR4 signal transduction pathway. Cytokine 2008; 42(2): 145-51.
[http://dx.doi.org/10.1016/j.cyto.2008.01.006] [PMID: 18304834]
[9]
Trentin-Sonoda M, da Silva RC, Kmit FV, et al. Knockout of toll-like receptors 2 and 4 prevents renal ischemia-reperfusion-induced cardiac hypertrophy in mice. PLoS One 2015; 10(10): e0139350.
[http://dx.doi.org/10.1371/journal.pone.0139350] [PMID: 26448184]
[10]
Morin SO, Poggi M, Alessi M-C, Landrier J-F, Nunès JA. Modulation of t cell activation in obesity. Antioxid Redox Signal 2017; 26(10): 489-500. Available from: http://www.liebertpub.com/doi/10.1089/ars.2016.6746
[http://dx.doi.org/10.1089/ars.2016.6746] [PMID: 27225042]
[11]
de la Maza MP, Estevez A, Bunout D, Klenner C, Oyonarte M, Hirsch S. Ventricular mass in hypertensive and normotensive obese subjects. Int J Obes Relat Metab Disord 1994; 18(4): 193-7. Available from: http://www.ncbi.nlm.nih.gov/pubmed/8044192
[PMID: 8044192]
[12]
Zhu L, Li C, Liu Q, Xu W, Zhou X. Molecular biomarkers in cardiac hypertrophy. J Cell Mol Med 2019; 23(3): 1671-7. Available from: http://doi.wiley.com/10.1111/jcmm.14129
[http://dx.doi.org/10.1111/jcmm.14129] [PMID: 30648807]
[13]
Nascimento-Sales M, Fredo-da-Costa I, Borges Mendes ACB, et al. Is the FVB/N mouse strain truly resistant to diet-induced obesity? Physiol Rep 2017; 5(9): 5.
[http://dx.doi.org/10.14814/phy2.13271] [PMID: 28483861]
[14]
Sartorelli DS, Franco LJ. Tendências do diabetes mellitus no Brasil: O papel da transição nutricional. Cad Saude Publica 2003; 19(Suppl. 1): S29-36.
[http://dx.doi.org/10.1590/S0102-311X2003000700004] [PMID: 12886433]
[15]
Wang Z, Li L, Zhao H, Peng S, Zuo Z. Chronic high fat diet induces cardiac hypertrophy and fibrosis in mice. Metabolism 2015; 64(8): 917-25. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0026049515001286
[http://dx.doi.org/10.1016/j.metabol.2015.04.010] [PMID: 25982698]
[16]
Zeyda M, Stulnig TM. Obesity, inflammation, and insulin resistance--a mini-review. Gerontology 2009; 55(4): 379-86. Available from: https://www.karger.com/Article/FullText/212758
[http://dx.doi.org/10.1159/000212758] [PMID: 19365105]
[17]
Ouchi N, Parker JL, Lugus JJ, Walsh K. Adipokines in inflammation and metabolic disease. Nat Rev Immunol 2011; 11(2): 85-97. Available from: http://www.nature.com/articles/nri2921
[http://dx.doi.org/10.1038/nri2921] [PMID: 21252989]
[18]
Tilg H, Moschen AR. Inflammatory mechanisms in the regulation of insulin resistance. Mol Med 2008; 14(3-4): 222-31. Available from: https://molmed.biomedcentral.com/articles/10.2119/2007-00119.Tilg
[http://dx.doi.org/10.2119/2007-00119.Tilg] [PMID: 18235842]
[19]
Uysal KT, Wiesbrock SM, Marino MW, Hotamisligil GS. Protection from obesity-induced insulin resistance in mice lacking TNF-α function. Nature 1997; 389(6651): 610-4. Available from: http://www.nature.com/articles/39335
[http://dx.doi.org/10.1038/39335] [PMID: 9335502]
[20]
Takeda K, Kaisho T, Akira S. Toll-like receptors. Annu Rev Immunol 2003; 21(1): 335-76. Available from: http://www.annualreviews.org/doi/10.1146/annurev.immunol.21.120601.141126
[http://dx.doi.org/10.1146/annurev.immunol.21.120601.141126] [PMID: 12524386]

Rights & Permissions Print Cite
Article Metrics
34
1
World Malaria Day
© 2024 Bentham Science Publishers | Privacy Policy