A Review: Computational approaches to design sgRNA of CRISPR-Cas9

(E-pub Abstract Ahead of Print)

Author(s): Mohsin Ali Nasir, Samia Nawaz, Jia Huang*

Journal Name: Current Bioinformatics


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Abstract:

Clustered regularly interspaced short palindromic repeats along with CRISPR-associated protein mechanisms preserve the memory of previous experiences with DNA invaders, in particular spacers that are embedded in CRISPR arrays between coordinate repeats. There has been a fast progression in the comprehension of this immune system and its implementations; however, there are numerous points of view that anticipate explanations to make the field an energetic research zone. The efficiency of CRISPR-Cas depends on well considered single guide RNA. For this purpose, many bioinformatics methods and tools were created to support the design of greatly active and precise single guide RNA. In-silico single guide RNA architecture is a crucial point for effective gene editing by means of the CRISPR technique. Persistent attempts are prepared to improve in-silico single guide RNA formulation by great on-target effectiveness and decreased off-target effects. This review offers a summary of the CRISPR computational tools to help different researchers to pick a specific tool for their work according to their pros and cons, along with new thoughts to make new computational tools to overcome all existing limitations.

Keywords: CRISPR-Cas9, sgRNA, Computational Tools, Off-Target Effects, On-target, Protein.

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Article Details

(E-pub Abstract Ahead of Print)
DOI: 10.2174/1574893616666210601105553
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