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Current Materials Science

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ISSN (Print): 2666-1454
ISSN (Online): 2666-1462

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Research Article

Estimation of Glucose and Galactose Content in a Natural Polysaccharide Isolated from Tubers of Colocasia Esculenta using a Validated RP-HPLC Method

Author(s): Harshal Ashok Pawar*, Pritam Dinesh Choudhary and Amit Jagannath Gavasane

Volume 15, Issue 1, 2022

Published on: 28 April, 2021

Page: [68 - 78] Pages: 11

DOI: 10.2174/2666145414666210428103241

Price: $65

Abstract

Background: Nowadays, natural polysaccharides are preferred over synthetic polymers because they are biocompatible, cheap, and easily available than synthetic ones. The polysaccharide obtained from the tuber of the Colocasia esculenta was reported to exhibit many pharmaceutical applications as an excipient.

Objective: The application of any polymer in formulating various dosage forms depends upon its qualitative and quantitative chemical composition. Therefore, in the current research work, the quantitative composition of the polysaccharide isolated from Colocasia esculenta was determined by the validated HPLC method.

Methods: RP-HPLC analysis was performed on an Agilent, Zorbax Eclipse XDB C8 column (250 mm × 4.6 mm, 5 μm) with the mobile phase consisting of citrate buffer of pH 5.5: Tetrahydrofuran: Acetonitrile (87.1:11.9:1 v/v) at a flow rate of 0.6 mL/min. UV detection was performed at 307 nm. The method was validated for various parameters as per ICH guidelines.

Results: The calibration curve constructed for glucose and galactose was found linear with correlation coefficients of 0.997 and 0.994, respectively. The system suitability parameters, such as theoretical plate, tailing factor, and relative standard deviation (RSD), were well within the limits. Solution stability data indicated that the solution was stable for 24 hours at 25°C. The watersoluble polysaccharide from Colocasia esculenta was found to contain glucose and galactose in the ratio 8:1.

Conclusion: The validated RP-HPLC method was found to be specific, linear, precise, and accurate and can be successfully used for simultaneous estimation of glucose and galactose content in the polysaccharides.

Keywords: Aldose derivatization, Colocasia esculenta, RP-HPLC, polysaccharides, glucose, galactose.

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[1]
Zatz JL, Kushla GP. Oral aqueous suspensions and gels. In: Pharmaceutical Dosage Forms: Disperse Systems New York: Marcel Dekker. 1989; 2: pp. 164-405.
[2]
Choudhary PD, Pawar HA. Recently investigated natural gums and mucilages as pharmaceutical excipients: an overview. J Pharm 2014; 2014: 204849.
[http://dx.doi.org/10.1155/2014/204849]
[3]
Boggs L, Cuendet LS, Ehrenthal I, Koch R, Smith F. Separation and identification of sugars using paper chromatography. Nature 1950; 166(4221): 520-1.
[http://dx.doi.org/10.1038/166520b0] [PMID: 14780127]
[4]
Bhushan R, Kaur S. TLC separation of some common sugars on silica gel plates impregnated with transition metal ions. Biomed Chromatogr 1997; 11(1): 59-60.
[http://dx.doi.org/10.1002/(SICI)1099-0801(199701)11:1<59:AID-BMC632>3.0.CO;2-Q] [PMID: 9051220]
[5]
Olechno JD, Carter SR, Edwards WT, Guillen DG. Developments in the chromatographic determination of carbohydrates. Anal Biotechnol Lab 1987; 5: 38.
[6]
Blanken WM, Bergh MLE, Koppen PL, van den Eijnden DH. High-pressure liquid chromatography of neutral oligosaccharides: effects of structural parameters. Anal Biochem 1985; 145(2): 322-30.
[http://dx.doi.org/10.1016/0003-2697(85)90369-0] [PMID: 4014663]
[7]
Karlsson H, Carlstedt I, Hansson GC. The use of gas chromatography and gas chromatography-mass spectrometry for the characterization of permethylated oligosaccharides with molecular mass up to 2300. Anal Biochem 1989; 182(2): 438-46.
[http://dx.doi.org/10.1016/0003-2697(89)90620-9] [PMID: 2610361]
[8]
Marko-Varga GA. Determination of mono- and oligosaccharides in fermentation broths by liquid chromatographic separation and amperometric detection using immobilized enzyme reactors and a chemically modified electrode. Anal Chem 1989; 61(8): 831-8.
[http://dx.doi.org/10.1021/ac00183a010] [PMID: 2719275]
[9]
Bai C, Ohrui H, Nishida Y, Meguro H. A fluorometric determination method for D,L configurations of per-O-methylated monosaccharides by anomeric 2-methyl-2-beta-naphthyl-1,3-benzodioxole 4-carboxylation and high-performance liquid chromatography. Anal Biochem 1997; 246(2): 246-52.
[http://dx.doi.org/10.1006/abio.1997.2016] [PMID: 9073363]
[10]
Wang WT, LeDonne NC Jr, Ackerman B, Sweeley CC. Structural characterization of oligosaccharides by high-performance liquid chromatography, fast-atom bombardment-mass spectrometry, and exoglycosidase digestion. Anal Biochem 1984; 141(2): 366-81.
[http://dx.doi.org/10.1016/0003-2697(84)90057-5] [PMID: 6496945]
[11]
Kwon H, Kim J. Determination of monosaccharides in glycoproteins by reverse-phase high-performance liquid chromatography 1993; 215(2): 243-52.
[http://dx.doi.org/10.1006/abio.1993.1582]
[12]
Hicks KB. High-performance liquid chromatography of carbhydrates. Adv Carbohydr Chem Biochem 1988; 46: 17.
[http://dx.doi.org/10.1016/S0065-2318(08)60164-X]
[13]
Verzele M, Simoens G, Van F. Acritical review of some liquid chromatography systems for separation of sugars. Chromatographia 1987; 23: 292.
[http://dx.doi.org/10.1007/BF02311783]
[14]
Akiyama T. Separation of neutral mono and oligosaccharides derivatised with ethyl p-aminobenzoate by high-performance liquid chromatography on an amine-bonded vinyl alcohol copolymer column. J Chromatogr A 1991; 588: 53-9.
[http://dx.doi.org/10.1016/0021-9673(91)85007-3]
[15]
Kerheve P, Charriere B, Gadel F, Chromatogr J. A determination of marine monosaccharides by high-pH anion-exchange chromatography with pulsed amperometric detection. J Chromatogr A 1995; 718(2): 283-9.
[16]
Wang WT, Erlansson K, Lindh F, Lundgren T, Zopf D. High-performance liquid chromatography of sialic acid-containing oligosaccharides and acidic monosaccharides. Anal Biochem 1990; 190(2): 182-7.
[http://dx.doi.org/10.1016/0003-2697(90)90178-C] [PMID: 2291464]
[17]
Sørensen SH, Proud FJ, Adam A, Rutgers HC, Batt RM. A novel HPLC method for the simultaneous quantification of monosaccharides and disaccharides used in tests of intestinal function and permeability. Clin Chim Acta 1993; 221(1-2): 115-25.
[http://dx.doi.org/10.1016/0009-8981(93)90026-Z] [PMID: 8149629]
[18]
Anumula KR. Quantitative determination of monosaccharides in glycoproteins by high-performance liquid chromatography with highly sensitive fluorescence detection. Anal Biochem 1994; 220(2): 275-83.
[http://dx.doi.org/10.1006/abio.1994.1338] [PMID: 7978269]
[19]
Rakotomanga S, Baillet A, Pellerin F, Baylocq-Ferrier D. Liquid chromatographic analysis of monosaccharides with phenylisocyanate derivatization. J Pharm Biomed Anal 1992; 10(8): 587-91.
[http://dx.doi.org/10.1016/0731-7085(92)80084-Z] [PMID: 1463793]
[20]
Formato M, Senes A, Soccolini F, Coinu R, Cherchi GM. A reversed phase HPLC method for the simultaneous determination of all monosaccharides contained in galactosaminoglycan isomers from human aorta proteoglycans. Carbohydr Res 1994; 255: 27-39.
[http://dx.doi.org/10.1016/S0008-6215(00)90969-8] [PMID: 8181011]
[21]
Zhang RE, Cao YL, Hearn MW. Synthesis and application of Fmoc-hydrazine for the quantitative determination of saccharides by reversed-phase high-performance liquid chromatography in the low and subpicomole range. Anal Biochem 1991; 195(1): 160-7.
[http://dx.doi.org/10.1016/0003-2697(91)90312-H] [PMID: 1888013]
[22]
Kerns RJ, Linhardt RJ. Separation of hydroxyl protected heparin derived disaccharides using reversed-phase high-performance liquid chromatography. J Chromatogr A 1995; 705(2): 369-73.
[http://dx.doi.org/10.1016/0021-9673(95)00293-V] [PMID: 7640772]
[23]
Yasuno S, Murata T, Kokubo K, Yamaguchi T, Kamei M. Two-mode analysis by High-performance liquid chromatography of ρ-Aminobenzoic Ethyl Ester-derivatized monosaccharides. Biosci Biotechnol Biochem 1997; 61(11): 1944-6.
[http://dx.doi.org/10.1271/bbb.61.1944] [PMID: 27396745]
[24]
Honda S, Akao E, Suzuki S, Okuda M, Kakehi K, Nakamura J. High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives. Anal Biochem 1989; 180(2): 351-7.
[http://dx.doi.org/10.1016/0003-2697(89)90444-2] [PMID: 2817366]
[25]
Honda S, Suzuki S, Nose A, Yamamoto K, Kakehi K. High-performance capillary zone electrophoresis of carbohydrates in the presence of alkaline earth metal ions. Carbohydr Res 1991; 215: 193.
[http://dx.doi.org/10.1016/0008-6215(91)84018-A]
[26]
Boban PT, Nambisan B, Sudhakaran PR. Hypolipidaemic effect of chemically different mucilages in rats: a comparative study. Br J Nutr 2006; 96(6): 1021-9.
[http://dx.doi.org/10.1017/BJN20061944] [PMID: 17181876]
[27]
Shah BN, Nayak BS, Bhatt SP, Jalalpure SS, Sheth AK. The anti-inflammatory activity of the leaves of Calocasia esculenta. Saudi Pharm J 2007; 15: 3-4.
[28]
Kirtikar K R, Basu B D. Indian medicinal plants. In: Dehradun: International Book distributors. 2005; 4: p. 261.
[29]
Kalariya M, Parmar S, Sheth N. Neuropharmacological activity of hydroalcoholic extract of leaves of Colocasia-esculenta. Pharm Biol 2010; 48(11): 1207-12.
[http://dx.doi.org/10.3109/13880201003586887] [PMID: 20818937]
[30]
Grindley BA, Omoruyi F, Asemota HN, Morrisona A. Carbohydrate digestion and intestinal ATPases in streptozotocin-induced diabetic rats fed extract of yam (Dioscorea cayenensis) or dasheen (Colocasia esculenta). Nutr Res 2002; 22: 333-41.
[http://dx.doi.org/10.1016/S0271-5317(01)00385-2]
[31]
Pawar HA, Kamat SR, Choudhary PD. An overview of natural polysaccharides as biological macromolecules: their chemical modifications and pharmaceutical applications. Biol Med 2015; 6: 224.
[http://dx.doi.org/10.4172/0974-8369.1000224]
[32]
ICH validation of analytical procedures: text and methodology Q2 (R1). Proceedings of International Conference on Harmonization 2005.
[33]
Pawar HA, Joshi PR. Development and validation of stability indicating RP-HPLC method for Estimation of satranidazole from its formulation. ISRN Chromatography 2014; 2014: 689218.
[http://dx.doi.org/10.1155/2014/689218]
[34]
Gomisa DB, Tamayoa DM, Alonso JM. Determination of monosaccharides in cider by reversed-phase liquid chromatography. Anal Chim Acta 2001; 436: 173-80.
[http://dx.doi.org/10.1016/S0003-2670(01)00889-3]

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