Potential Application of CRISPR/Cas9 System to Engineer Abiotic Stress Tolerance in Plants

(E-pub Abstract Ahead of Print)

Author(s): Temoor Ahmed, Muhammad Shahid*, Muhammad Noman, Sher Muhammad, Muhammad Tahir ul Qamar, Md. Arshad Ali, Awais Maqsood, Rahila Hafeez, Solabomi Olaitan Ogunyemi, Bin Li

Journal Name: Protein & Peptide Letters


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Abstract:

Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review highlighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.

Keywords: CRISPR/Cas9, abiotic stresses, genome editing, crop improvement, transcription, sgRNA.

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Article Details

(E-pub Abstract Ahead of Print)
DOI: 10.2174/0929866528666210218220138
Price: $95