Pharmacokinetics and Efficacy of Ceftriaxone in Staphylococcal Mastitis in Crossbred Cows Following Single Intravenous Administration

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Author(s): Rinku Buragohain, Tapas Kumar Sar*, Ujjwal Biswas, Indranil Samanta, Tapan Kumar Mandal

Journal Name: Current Drug Metabolism

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Background:Clinical mastitis is an important production disease of dairy animals causing significant economic loses.

Objective: Disposition kinetics of ceftriaxone was conducted in healthylactating and staphylococcal mastitic crossbred cows in field condition following single dose intravenous administration of only ceftriaxone.

Method:A single dose of ceftriaxone at 20 mg kg-1 body weight was administered intravenously through jugular vein to six clinically healthy and six mastitic crossbred cowsafter proper diagnosis and three mastitic cows remained untreated (positive control).Blood and milk samples were collected at 0 (pre dosing), 5, 15, 30 min, and 1, 24, 48, 72, 96 and 120 h post drug administration and analyzed for ceftriaxone and its active metabolite (ceftizoxime) byhigh performance liquid chromatography.

Results: Ceftriaxone achieved a peak mean plasma concentration of 131.67±1.83 µg mL-1 at 5 min, which decreased sharply until 1 h (35.56±0.44 µg mL-1) and was below detection limit at 24 h post drug administration in mastitic crossbred cows. On the other hand, ceftizoxime (active metabolite of ceftriaxone) achieved a peak level of 55.42±3.34 µg mL-1 at 72 h and could not be detected at 120 h post drug administration in the milk of thosemastitic crossbred cows. The Staphylococcus aureus colony count in mastitic crossbred cows was 49.33±6.55 × 105 c.f.u./mL and the lowest colony count was achieved at 72 h with no colony at 120 h post drug administration. All the staphylococcal mastitis affected crossbred cows were cured at day 5.

Conclusion:Ceftriaxone may be effective in treatment of staphylococcal mastitis in crossbred cows following single dose intravenous administration at 20 mg kg-1 body weight.

Keywords: Ceftriaxone, Ceftizoxime, Disposition kinetics, Staphylococcus aureus, Mastitis, Crossbred cow

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1389200222666210210113641
Price: $95