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Current Chromatography

Editor-in-Chief

ISSN (Print): 2213-2406
ISSN (Online): 2213-2414

Research Article

High-Throughput Determination of Vitamins (B1, B2, B3, B5, B6, B7, and B9) using UPLC-ESI-MS

Author(s): Abdullah S. Al-Dosseri, Mohd A. Alam* and Fahad I. Al-Jenoobi

Volume 8, Issue 1, 2021

Published on: 02 February, 2021

Page: [1 - 9] Pages: 9

DOI: 10.2174/2213240608666210203112120

Price: $65

Abstract

Background: To prevent vitamin deficiencies or to restore their levels, multi-vitamin formulations are indicated for the elderly, adults, children, and infants.

Objective: To provide a valid high-throughput method for simultaneous determination of vitamins in multi-vitamin formulations.

Methods: A high-throughput UPLC-ESI-MS method was developed and validated for simultaneous determination of vitamins B1, B2, B3, B5, B6, B7, and B9. Analytes were eluted on an Acquity UPLC® BEH C18 1.7 μm, 2.1 x 50 mm column at 40 ± 5°C. Mobile phase containing acetonitrile (0.1% formic acid) and water (0.1% formic acid) in 30:70% ratio was pumped at 300 μL/min under isocratic control. Protonated ions of vitamin B1, B2, B3, B5, B6, B7, and B9 were monitored in single ion recording mode, using an electrospray ionization probe in positive mode.

Results: The m/z ratios of positive ions of vitamin B1, B2, B3, B5, B6, B7, and B9 were 265.1, 377.2, 122.95, 220, 169.98, 244.9, and 442.1, respectively. The calibration curve of different linearity range (ng/ml) was prepared for each vitamin. Linearity range for vitamin B1, B2, B3, B5, B6, B7, and B9 were 60-1000, 25-1000, 75-5000, 30-1000, 30/1000, 25/1000 and 30-900 25-1000 ng/mL, respectively. Coefficient of variation for intra-day and inter-day precision for vitamin B1, B2, B3, B5, B6, B7, and B9 at the middle and higher limit of quantitation were less than 15%.

Conclusion: The method was successfully developed and validated, and three different brands of multi-vitamin tablets were assayed for water-soluble vitamins.

Keywords: Multi-vitamin tablets, riboflavin, thiamine, folic acid, nicotinamide, pyridoxine, biotin, pantothenic acid.

Graphical Abstract
[1]
Kennedy, D.O.B. Vitamins and the brain: Mechanisms, dose and efficacy--A Review. Nutrients, 2016, 8(2), 68.
[http://dx.doi.org/10.3390/nu8020068] [PMID: 26828517]
[2]
Kimura, N.; Fukuwatari, T.; Sasaki, R.; Hayakawa, F.; Shibata, K. Vitamin intake in Japanese women college students. J. Nutr. Sci. Vitaminol. (Tokyo), 2003, 49(3), 149-155.
[http://dx.doi.org/10.3177/jnsv.49.149] [PMID: 12953791]
[3]
Fratoni, V.; Brandi, M.L. B vitamins, homocysteine and bone health. Nutrients, 2015, 7(4), 2176-2192.
[http://dx.doi.org/10.3390/nu7042176] [PMID: 25830943]
[4]
Brito, A.; Hertrampf, E.; Olivares, M.; Gaitán, D.; Sánchez, H.; Allen, L.H.; Uauy, R. Folate, vitamin B12 and human health. Rev. Med. Chil., 2012, 140(11), 1464-1475.
[http://dx.doi.org/10.4067/S0034-98872012001100014] [PMID: 23677195]
[5]
Briani, C.; Dalla Torre, C.; Citton, V.; Manara, R.; Pompanin, S.; Binotto, G.; Adami, F. Cobalamin deficiency: clinical picture and radiological findings. Nutrients, 2013, 5(11), 4521-4539.
[http://dx.doi.org/10.3390/nu5114521] [PMID: 24248213]
[6]
Qin, L.L.; Lu, F.G.; Yang, S.H.; Xu, H.L.; Luo, B.A. Does maternal vitamin d deficiency increase the risk of preterm birth: A meta-analysis of observational studies. Nutrients, 2016, 8(5), E301.
[http://dx.doi.org/10.3390/nu8050301] [PMID: 27213444]
[7]
Moser, M.A.; Chun, O.K. Vitamin C and heart health: A review based on findings from epidemiologic studies. Int. J. Mol. Sci., 2016, 17(8), E1328.
[http://dx.doi.org/10.3390/ijms17081328] [PMID: 27529239]
[8]
Chawla, J.; Kvarnberg, D. Hydrosoluble vitamins. Handb. Clin. Neurol., 2014, 120, 891-914.
[http://dx.doi.org/10.1016/B978-0-7020-4087-0.00059-0] [PMID: 24365359]
[9]
Konings, E.J. Committee on Food Nutrition. Water-soluble vitamins. J. AOAC Int., 2006, 89(1), 285-288.
[http://dx.doi.org/10.1093/jaoac/89.1.285] [PMID: 16512258]
[10]
Ursić, S.; Luterotti, S.; Ljubas, D. Partial multifactorial design in modelling of UV-spectrometric assays of ascorbic acid with nitrosobenzene and p-nitro-nitrosobenzene. Fresenius J. Anal. Chem., 2001, 369(7-8), 719-726.
[http://dx.doi.org/10.1007/s002160100702] [PMID: 11371079]
[11]
Diaz, A.N.; Paniagua, A.G.; Sánchez, F.G. Thin-layer chromatography and fibre-optic fluorimetric quantitation of thiamine, riboflavin and niacin. J. Chromatogr. A, 1993, 655(1), 39-43.
[http://dx.doi.org/10.1016/0021-9673(93)87007-9]
[12]
Moreno, P.; Salvadó, V. Determination of eight water- and fat-soluble vitamins in multi-vitamin pharmaceutical formulations by high-performance liquid chromatography. J. Chromatogr. A, 2000, 870(1-2), 207-215.
[http://dx.doi.org/10.1016/S0021-9673(99)01021-3] [PMID: 10722078]
[13]
Chatzimichalakis, P.F.; Samanidou, V.F.; Papadoyannis, I.N. Development of a validated liquid chromatography method for the simultaneous determination of eight fat-soluble vitamins in biological fluids after solid-phase extraction. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2004, 805(2), 289-296.
[http://dx.doi.org/10.1016/j.jchromb.2004.03.009] [PMID: 15135103]
[14]
Klejdus, B.; Petrlova, J.; Potesil, D.; Adam, V.; Mikelova, R.; Vacek, J.; Kizek, R.; Kuban, V. Simultaneous determination of water-and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Anal. Chim. Acta, 2004, 520(1-2), 57-67.
[http://dx.doi.org/10.1016/j.aca.2004.02.027]
[15]
Zafra-Gómez, A.; Garballo, A.; Morales, J.C.; García-Ayuso, L.E. Simultaneous determination of eight water-soluble vitamins in supplemented foods by liquid chromatography. J. Agric. Food Chem., 2006, 54(13), 4531-4536.
[http://dx.doi.org/10.1021/jf060346e] [PMID: 16786994]
[16]
Taguchi, K.; Fukusaki, E.; Bamba, T. Simultaneous analysis for water- and fat-soluble vitamins by a novel single chromatography technique unifying supercritical fluid chromatography and liquid chromatography. J. Chromatogr. A, 2014, 1362, 270-277.
[http://dx.doi.org/10.1016/j.chroma.2014.08.003] [PMID: 25200530]
[17]
Cellar, N.A.; McClure, S.C.; Salvati, L.M.; Reddy, T.M. A new sample preparation and separation combination for precise, accurate, rapid, and simultaneous determination of vitamins B1, B2, B3, B5, B6, B7, and B9 in infant formula and related nutritionals by LC-MS/MS. Anal. Chim. Acta, 2016, 934, 180-185.
[http://dx.doi.org/10.1016/j.aca.2016.05.058] [PMID: 27506358]
[18]
Li, H.B.; Chen, F. Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection. J. Sep. Sci., 2001, 24(4), 271-274.
[http://dx.doi.org/10.1002/1615-9314(20010401)24:4<271::AID-JSSC271>3.0.CO;2-L]
[19]
Kakitani, A.; Inoue, T.; Matsumoto, K.; Watanabe, J.; Nagatomi, Y.; Mochizuki, N. Simultaneous determination of water-soluble vitamins in beverages and dietary supplements by LC-MS/MS. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 2014, 31(12), 1939-1948.
[http://dx.doi.org/10.1080/19440049.2014.977965] [PMID: 25325190]
[20]
Chen, Z.; Chen, B.; Yao, S. High-performance liquid chromatography/electrospray ionization-mass spectrometry for simultaneous determination of taurine and 10 water-soluble vitamins in multivitamin tablets. Anal. Chim. Acta, 2006, 569(1), 169-175.
[http://dx.doi.org/10.1016/j.aca.2006.03.099]
[21]
Patil, S.S.; Srivastava, A.K. Development and validation of a liquid chromatography method for the simultaneous determination of eight water-soluble vitamins in multivitamin formulations and human urine. J. AOAC Int., 2013, 96(6), 1273-1280.
[http://dx.doi.org/10.5740/jaoacint.12-208] [PMID: 24645504]
[22]
Nurit, E.; Lyan, B.; Piquet, A.; Branlard, G.; Pujos-Guillot, E. Development of a LC-MS/MS method for the simultaneous screening of seven water-soluble vitamins in processing semi-coarse wheat flour products. Anal. Bioanal. Chem., 2015, 407(12), 3471-3479.
[http://dx.doi.org/10.1007/s00216-015-8553-1] [PMID: 25701425]
[23]
Gentili, A.; Caretti, F.; D’Ascenzo, G.; Marchese, S.; Perret, D.; Di Corcia, D.; Rocca, L.M. Simultaneous determination of water-soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun. Mass Spectrom., 2008, 22(13), 2029-2043.
[http://dx.doi.org/10.1002/rcm.3583] [PMID: 18512845]
[24]
Leporati, A.; Catellani, D.; Suman, M.; Andreoli, R.; Manini, P.; Niessen, W.M. Application of a liquid chromatography tandem mass spectrometry method to the analysis of water-soluble vitamins in Italian pasta. Anal. Chim. Acta, 2005, 531(1), 87-95.
[http://dx.doi.org/10.1016/j.aca.2004.10.006]
[25]
Al-Othman, Z.A.; Al-Warthan, A.; Ali, I. Advances in enantiomeric resolution on monolithic chiral stationary phases in liquid chromatography and electrochromatography. J. Sep. Sci., 2014, 37(9-10), 1033-1057.
[http://dx.doi.org/10.1002/jssc.201301326] [PMID: 24634395]
[26]
Ali, I.; Al-Othman, Z.A.; Al-Warthan, A.; Aboul-Enein, H.Y. Recent trends in chiral separations by nano liquid chromatography and nano capillary electrophoresis. Curr. Chromatogr., 2014, 1, 81-89.
[http://dx.doi.org/10.2174/2213240601666140301001948]
[27]
Ali, I.; Aboul-Enein, H.Y. Enantioseparation of some clinically used drugs by HPLC using cellulose Tris (3,5-dichlorophenylcarbamate) chiral stationary phase. Biomed. Chromatogr., 2003, 17(2-3), 113-117.
[http://dx.doi.org/10.1002/bmc.220] [PMID: 12717799]
[28]
Edwards, M.; Boswell, H.; Górecki, T. Comprehensive multidimensional chromatography. Curr. Chromatogr., 2015, 2(2), 80-109.
[http://dx.doi.org/10.2174/2213240602666150722232236]
[29]
Anonymous, Validation of analytical procedures: text and methodology Q2 (R1): ICH harmonised tripartite guideline. 2005.http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf
[30]
Anonymous, Water-Soluble vitamins tablets official monograph. United States pharmacopoeia and national formulary, USP40–NF35. Rockville (MD); USP Convention: 2017a, pp. 7412.
[31]
Anonymous. Water-soluble vitamins with minerals tablets official monograph. United States pharmacopoeia and national formulary, USP40–NF35. Rockville (MD); USP Convention: 2017b, pp. 7451.
[32]
Anonymous. Oil- and water-soluble vitamins tablets official monograph. United States pharmacopoeia and national formulary, USP40–NF35. Rockville (MD); USP Convention: 2017c, pp. 7318.
[33]
Anonymous. Oil- and water-soluble vitamins with minerals tablets official monograph. United States pharmacopoeia and national formulary, USP40–NF35. Rockville (MD); USP Convention: 2017d, pp. 7375.

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