Background: In new modern science Nanotechnology is an emerging field for the researchers. Nanotechnology
deals with the Nanoparticles. These Nanoparticles have a size of 1-100 nm in one dimension which are important part of
biomedical science and medical chemistry, atomic physics, and all other known fields. Nanoparticles are used in broad
range due to its small size, orientation, physical properties.
Objectives: This study was designed to synthesized Silver nanoparticles using Azadirachta indica (Neem) leaf extract and
evaluate biomedical application.
Methods: Aqueous extract of Azadirachta indica is used for the synthesis of AgNPs. 1ml of the extract was added to (30-
60) ml of 1mM solution of silver nitrate drop by drop. Silver nanoparticle was characterized by UV-Visible Spectroscopy,
Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering Spectroscopy, X-ray Diffraction, Transmission
Electron Microscopy, Antibacterial activity studies.
Results: The Biological synthesis of Silver nanoparticles (AgNPs) was done by using the aqueous solution of Azadirachta
indica leaf extract and AgNO3. A fixed ratio of plant extract to metal ion was used to prepare AgNPs and the formation of
the nanoparticles was observed by the color change. The nanoparticles were characterized by UV-vis Spectrophotometer,
FTIR, DLS, XRD and TEM. The nanoparticles were found have the size ranges from 30-60 nm. The biosynthesized silver
nanoparticles had bactericidal effect against antibiotic resistance pathogenic microorganisms such as Bascillus subtilis,
pseudomonas aeruginosa and Bascillus cereus.
Conclusion: Silver nanoparticles were synthesized by Azadirachta indica leaf extract can be used as a therapeutic candidate
for biomedical applications.