In vitro validation of camphene as a potential antiviral agent against betanodavirus causing viral nervous necrosis in barramundi

(E-pub Abstract Ahead of Print)

Author(s): Ruby Singh, Prachi Srivastava*, Deepika Anand, Pani K. Prasad

Journal Name: Anti-Infective Agents
Formerly Anti-Infective Agents in Medicinal Chemistry

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Introduction: Viral infections are major threat to aquaculture industry throughout the world. Betanodavirus is one of the most infectious virus that causes highest mortality in larval and juvenile stages of Lates calcarifer commonly known as Barramundi.

Methods: It is a single stranded positive sense RNA virus and causes viral nervous necrosis (VNN). VNN is caused by RNA virus which gets transmitted both horizontally and vertically so the most effective method against this virus is to vaccinate the fish, however vaccination becomes difficult since the disease is associated with the outbreaks in larval and juvenile stages which are not that much immunocompetent. In our previous in silico studies we proved the stability of camphene as a better phytochemical agent.

Results: In continuation to prove the authenticity of Camphene as potential antiviral agent against betanodavirus, its in vitro validation was performed. Sea bass kidney cell line (SISK) was selected for carrying out the in vitro studies and cytotoxicity studies of Camphene in the SISK was was done by MTT assay. Based on the analysis of MTT assay different dosage of camphene were selected viz.,(0.2, 0.5, 1, 1.5, 2, 2.5, 5, 10, 20, 30 μg/ml). The SISK cells were infected with virus inoculum (200μl). Further the antiviral activity of Camphene on infected SISK cells by Betanodavirus was elucidated with the help of quantitative Real time PCR(qPCR) on the 3rd and 5th day of infection.

Conclusion: Analysis of results depictecd that the dose of camphene 2 to 10 μg/ml is the safest dose against Betanodavirus. Hence this is aptly revealed that camphene can be used as potential antiviral agent against Betanodavirus.

Keywords: Viral nervous necrosis, Barramundi, Phytochemical, Molecular docking, Molecular Dynamic simulation, Camphene.

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(E-pub Abstract Ahead of Print)
DOI: 10.2174/2211352519999210111223529
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