Background: Food-drug interactions may lead to suppression or induction drug metabolizing enzymes.
Pomegranate is a commonly used fruit in folk medicine all over the world. Data concerning the effect of pomegranate on
the activity of UDP-glucuronosyltransferases (UGTs) is scarce.
Objective: The purpose of this work was to investigate the effect of pomegranate juice ingestion on the transcription of
ugt2b1, ugt2a3, and ugt1a9 in the liver and small intestine of male mice.
Methods: Pomegranate juice was administered to 10 male mice for 14 days in drinking bottles instead of water. Ten
control mice received water in the drinking bottles. On the 15th day, the mice were sacrificed and the liver and the small
intestine were removed. The small intestine was divided into 3 parts. Total mRNA was extracted from samples of these
specimens, and cDNA was synthesized by quantitative real-time polymerase chain reaction (RT-PCR) using specific
primers for each ugt gene.
Results: The ugt1a9 mRNA level was reduced by 2.25-fold in the liver and by 6-, 1.5-, and 3-folds in the first, second and
third part of the small intestine, respectively. The ugt2b1 mRNA level in the liver and the third part of the small intestine
was not affected, while it was reduced by 3.7- and 3-folds in the first and second parts of the small intestine, respectively.
The ugt2a3 mRNA level was not affected in the liver and the 3 parts of the small intestine.
Conclusions: Some ugt mRNA levels may be reduced by the ingestion of pomegranate juice, which may reduce the
metabolism of their drug substrates. The consequences may be accumulation of such drugs in the body and enhanced