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Current Nanomedicine

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ISSN (Print): 2468-1873
ISSN (Online): 2468-1881

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Research Article

Evaluation of Biological Effects and Toxicity of Cetyltrimethylammonium Bromide Stabilized Silver Nanoparticles and Cetyltrimethylammonium Bromide Alone Following Intravenous Injection in Mice

Author(s): Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, Neha Hebalkar, Rakesh K. Sharma, Shantveer G. Uppin and Kaiser Jamil*

Volume 11, Issue 1, 2021

Published on: 07 December, 2020

Page: [70 - 80] Pages: 11

DOI: 10.2174/2468187310666201207205245

Price: $65

Abstract

Background: The exciting benefits of Silver nanoparticles (AgNPs) in the biomedical field necessitate generating knowledge on the safety concerns which have been raised over the applications of these NPs.

Objective: To understand the biological effects and mechanism of toxicity induction of Cetyltrimethylammonium bromide (CTAB) stabilized AgNPs as well as CTAB alone in mice following intravenous injection.

Methods: The investigations were carried out by measuring hematological and serum biochemical parameters, oxidative stress, genotoxicity, and histopathology.

Results: AgNPs’ treatment was found to induce a marked decrease (p<0.05) in platelet and lymphocyte count, Serum glutamate oxaloacetate transaminase (SGOT), and an increase (p<0.05) in granulocytes count and Serum glutamate pyruvate transaminase (SGPT) whereas CTAB treatment-induced a decrease in platelet count. The decrease in glutathione (GSH) and an increase in lipid peroxidation (LPO) levels in the liver, spleen, and kidney of mice suggest the potential role of AgNPs in inducing oxidative stress. Genotoxicity was apparent from the increased comet parameters and micronuclei formation observed in the liver, spleen, and kidney of mice treated with AgNPs and CTAB. Histological examination in mice treated with AgNPs and CTAB showed diffused venous congestion and focal venous congestion respectively in the liver, while mild red pulp congestion in the spleen and acute tubular necrosis in the kidney were also observed.

Conclusion: There is a need to develop methods to dissolve the toxicity of CTAB, thereby it inducing relatively low or no toxicity without compromising the stability of nanosilver formation. Such insights are believed to be fundamental in the synthesis of high-performance AgNPs demonstrated for outstanding biomedical applications.

Keywords: Silver nanoparticles, Hemocompatibility, Oxidative stress, Genotoxicity, Histopathology, Acute toxicity.

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[1]
Burdușel AC, Gherasim O, Grumezescu AM, Mogoantă L, Ficai A, Andronescu E. Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials (Basel) 2018; 8(9): 681.
[http://dx.doi.org/10.3390/nano8090681] [PMID: 30200373]
[2]
Liu J, Wang Z, Liu FD, Kane AB, Hurt RH. Chemical transformations of nanosilver in biological environments. ACS Nano 2012; 6(11): 9887-99.
[http://dx.doi.org/10.1021/nn303449n] [PMID: 23046098]
[3]
Rai M, Ingle AP, Gupta I, Brandelli A. Bioactivity of noble metal nanoparticles decorated with biopolymers and their application in drug delivery. Int J Pharm 2015; 496(2): 159-72.
[http://dx.doi.org/10.1016/j.ijpharm.2015.10.059] [PMID: 26520406]
[4]
Khoza P, Ndhundhuma I, Karsten A, Nyokong T. Photodynamic Therapy Activity of Phthalocyanine-Silver Nanoparticles on Melanoma Cancer Cells. J Nanosci Nanotechnol 2020; 20(5): 3097-104.
[http://dx.doi.org/10.1166/jnn.2020.17398] [PMID: 31635653]
[5]
Akbarzadeh Khiavi M, Safary A, Barar J, et al. PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells. Bioimpacts 2020; 10(1): 27-36.
[http://dx.doi.org/10.15171/bi.2020.04] [PMID: 31988854]
[6]
Akter M, Sikder MT, Rahman MM, et al. A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J Adv Res 2017; 9: 1-16.
[http://dx.doi.org/10.1016/j.jare.2017.10.008] [PMID: 30046482]
[7]
Fei X, Ma X, Fang G, Chong Y, Tian X, Ge C. Antimicrobial Peptide-Templated Silver Nanoclusters with Membrane Activity for Enhanced Bacterial Killing. J Nanosci Nanotechnol 2020; 20(3): 1425-33.
[http://dx.doi.org/10.1166/jnn.2020.17161] [PMID: 31492303]
[8]
Pinzaru I, Coricovac D, Dehelean C, et al. Stable PEG-coated silver nanoparticles - A comprehensive toxicological profile. Food Chem Toxicol 2018; 111: 546-56.
[PMID: 29191727]
[9]
Kawata K, Osawa M, Okabe S. In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol 2009; 43(15): 6046-51.
[PMID: 19731716]
[10]
Yang L, Kuang H, Zhang W, Aguilar ZP, Wei H, Xu H. Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice. Sci Rep 2017; 7(1): 3303.
[http://dx.doi.org/10.1038/s41598-017-03015-1] [PMID: 28607366]
[11]
Sangeetha A, Samyuktha L, Kaiser J, Atya K, Swamy AVN, Harivardhan Reddy L. Biophysical, biopharmaceutical and toxicological significance of biomedical nanoparticles. RSC Advances 2015; 5: 47830-59.
[http://dx.doi.org/10.1039/C5RA05889A]
[12]
Sangeetha A, Samyuktha L, Swamy AVN, et al. Biological interactions in vitro of Zinc oxide nanoparticles of different characteristics. Mater Res Express 2014; 1035041
[http://dx.doi.org/10.1088/2053-1591/1/3/035041]
[13]
Sangeetha A, Samyuktha L, Atya K, et al. Route of administration induced in vivo effects and toxicity responses of Zinc Oxide nanorods at molecular and genetic levels. Int J Nanodimens 2018; 9: 158-69.
[14]
Sangeetha A, Samyuktha L, Atya K, Neha H, Sharma RK, Shantveer G. In Vivo Interactions of Nanosized Titania Anatase and Rutile Particles Following Oral Administration Nano Prog 2020; 2: 11-20.
[15]
Jimeno-Romero A, Oron M, Cajaraville MP, Soto M, Marigómez I. Nanoparticle size and combined toxicity of TiO2 and DSLS (surfactant) contribute to lysosomal responses in digestive cells of mussels exposed to TiO2 nanoparticles. Nanotoxicology 2016; 10(8): 1168-76.
[http://dx.doi.org/10.1080/17435390.2016.1196250] [PMID: 27241615]
[16]
Khan MN, Bashir O, Khan TA. AL-Thabaiti SA, Khan Z. CTAB capped synthesis of bio-conjugated silver nanoparticles and their enhanced catalytic activities. J Mol Liq 2018; 258: 133-41.
[http://dx.doi.org/10.1016/j.molliq.2018.02.130]
[17]
Gong T, Goh D, Olivo M, Yong KT. In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers. Beilstein J Nanotechnol 2014; 5: 546-53.
[http://dx.doi.org/10.3762/bjnano.5.64] [PMID: 24991490]
[18]
Jia YP, Shi K, Liao JF, et al. Effects of Cetyltrimethylammonium Bromide on the Toxicity of Gold Nanorods Both In Vitro and In Vivo: Molecular Origin of Cytotoxicity and Inflammation. Small Methods 2020; 4 : 1900799 .
[19]
Adam JK, Naidua KSB. Silver nanoparticles in biomedical applications: Recent Advances and perspectives. Nanomedicine and Nanotechnology in Health Care 10th International Conference. Bangkok, Thailand. 2016. July 25-27;
[20]
Janardhan R, Karuppaiah M, Hebalkar N, Rao TN. Synthesis and surface chemistry of nano silver particles. Polyhedron 2009; 28: 2522-30.
[http://dx.doi.org/10.1016/j.poly.2009.05.038]
[21]
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-8.
[PMID: 13967893]
[22]
Laughton MJ, Halliwell B, Evans PJ, Hoult JR. Antioxidant and pro-oxidant actions of the plant phenolics quercetin, gossypol and myricetin. Effects on lipid peroxidation, hydroxyl radical generation and bleomycin-dependent damage to DNA. Biochem Pharmacol 1989; 38(17): 2859-65.
[http://dx.doi.org/10.1016/0006-2952(89)90442-5] [PMID: 2476132]
[23]
Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000; 35(3): 206-21.
[http://dx.doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J] [PMID: 10737956]
[24]
Khaitan D, Chandna S, Arya MB, Dwarakanath BS. Differential mechanisms of radiosensitization by 2-deoxy-D-glucose in the monolayers and multicellular spheroids of a human glioma cell line. Cancer Biol Ther 2006; 5(9): 1142-51.
[http://dx.doi.org/10.4161/cbt.5.9.2986] [PMID: 16855378]
[25]
Countryman PI, Heddle JA. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat Res 1976; 41(2-3): 321-32.
[http://dx.doi.org/10.1016/0027-5107(76)90105-6] [PMID: 796719]
[26]
Tiwari DK, Jin T, Behari J. Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats. Toxicol Mech Methods 2011; 21(1): 13-24.
[http://dx.doi.org/10.3109/15376516.2010.529184] [PMID: 21080782]
[27]
Guclu E, Durmaz Y, Karabay O. Effect of severe sepsis on platelet count and their indices. Afr Health Sci 2013; 13(2): 333-8.
[http://dx.doi.org/10.4314/ahs.v13i2.19] [PMID: 24235932]
[28]
Wen H, Dan M, Yang Y, et al. Acute toxicity and genotoxicity of silver nanoparticle in rats. PLoS One 2017; 12(9): e0185554.
[http://dx.doi.org/10.1371/journal.pone.0185554] [PMID: 28953974]
[29]
Saquib Q, Al-Khedhairy AA, Siddiqui MA, Abou-Tarboush FM, Azam A, Musarrat J. Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells. Toxicol In Vitro 2012; 26(2): 351-61.
[http://dx.doi.org/10.1016/j.tiv.2011.12.011] [PMID: 22210200]
[30]
Krishnaraj C, Harper SL, Yun S-I. In Vivo toxicological assessment of biologically synthesized silver nanoparticles in adult Zebrafish (Danio rerio). J Hazard Mater 2016; 301: 480-91.
[http://dx.doi.org/10.1016/j.jhazmat.2015.09.022] [PMID: 26414925]
[31]
Nakata K, Tsuchido T, Matsumura Y. Antimicrobial cationic surfactant, cetyltrimethylammonium bromide, induces superoxide stress in Escherichia coli cells. J Appl Microbiol 2011; 110(2): 568-79.
[http://dx.doi.org/10.1111/j.1365-2672.2010.04912.x] [PMID: 21176007]
[32]
Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V. A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol 2010; 40(4): 328-46.
[http://dx.doi.org/10.3109/10408440903453074] [PMID: 20128631]
[33]
Ramachandran R, Krishnaraj C, Kumar VKA, Harper SL, Kalaichelvan TP, Yun SI. In vivo toxicity evaluation of biologically synthesized silver nanoparticles and gold nanoparticles on adult zebrafish: a comparative study 3 Biotech 2018; 8: 441.
[34]
Choi HS, Liu W, Misra P, et al. Renal clearance of quantum dots. Nat Biotechnol 2007; 25(10): 1165-70.
[http://dx.doi.org/10.1038/nbt1340] [PMID: 17891134]
[35]
Choi CH, Zuckerman JE, Webster P, Davis ME. Targeting kidney mesangium by nanoparticles of defined size. Proc Natl Acad Sci USA 2011; 108(16): 6656-61.
[http://dx.doi.org/10.1073/pnas.1103573108] [PMID: 21464325]
[36]
Guo H, Zhang J, Boudreau M, et al. Intravenous administration of silver nanoparticles causes organ toxicity through intracellular ROS-related loss of inter-endothelial junction. Part Fibre Toxicol 2016; 13: 21.
[http://dx.doi.org/10.1186/s12989-016-0133-9] [PMID: 27129495]
[37]
Wei L, Lu J, Xu H, Patel A, Chen ZS, Chen G. Silver nanoparticles: synthesis, properties, and therapeutic applications. Drug Discov Today 2015; 20(5): 595-601.
[http://dx.doi.org/10.1016/j.drudis.2014.11.014] [PMID: 25543008]
[38]
Lee SH, Jun BH. Silver Nanoparticles: Synthesis and Application for Nanomedicine. Int J Mol Sci 2019; 20(4): 865.
[http://dx.doi.org/10.3390/ijms20040865] [PMID: 30781560]

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