Background: Thrombolysis and endovascular thrombectomy acted as two mainly therapeutic strategies for the
ischemic stroke in clinic. However, reperfusion injury caused oxidative stress leading overproduction of reactive oxygen
species, mitochondrial dysfunction and subsequent cell death.
Methods: We designed and synthesized two tetramethylpyrazine-nitrone derivatives (T-003 and T-005) and investigated
their abilities for scavenging free radicals and protective effects as well as neurite outgrowth promotion in vitro.
Results and Discussion: Both of them showed potent radical-scavenging activity and neuroprotective effects against iodoacetic
acid-induced cells injury. Furthermore, T-003 and T-005 significantly promoted neurite outgrowth in PC12 cells.
Conclusion: Our results suggest that compound T-003 and T-005 could be potent antioxidants for the treatment of neurological
disease, particularly ischemic stroke.