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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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Research Article

Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats’ Sperm Quality and Testicular Histology

Author(s): Castillo-Maldonado Irais, Borjón-Ríos Claudia, Pedroza-Escobar David*, Sharma Ashutosh, García-Garza Rubén, Ramírez-Moreno Agustina, Vega-Menchaca M. del Carmen, Rivera-Guillén Mario-Alberto and Serrano-Gallardo Luis-Benjamín*

Volume 22, Issue 3, 2021

Published on: 30 July, 2020

Page: [400 - 407] Pages: 8

DOI: 10.2174/1389201021666200730145621

open access plus

Abstract

Background: The world's population is still growing, having an impact on the environment and the economic growth of developing countries; so that, there is a particular interest in the development of new fertility control methods, focused on male contraception.

Objective: The objective of this study was to evaluate the effect of methanolic extracts of leaf and fruit of Azadirachta indica on sperm quality and testicular histology of Long Evans rats.

Methods: Antifertility effects of a methanolic leaf and fruit extracts of A. indica on 24 male rats were investigated. The animals were randomly divided into two control groups and four treatment groups (n=4). Doses of the leaf and fruit extract were given at concentrations of 100 and 200 μg mL-1.

Results: A significant decrease in the viability of sperm cells was observed. The leaf extract at a concentration of 200 μg mL-1 inhibited cell viability compared to the negative control (p< 0.001). The percentage of abnormal cells in leaf extract was shown in 100 and 200 μg mL-1, the conditions at which a higher percentage of morphological irregularities of observed (15% and 16% respectively). The results show that there was cellular detachment in the seminiferous epithelium in the experimental groups treated with methanolic extracts. Sperm death was observed without decreasing the number of sperm.

Conclusion: The methanolic extracts of Azadirachta indica have a modulating effect on the spermatogenesis of experimental rats through sperm morphological alterations.

Keywords: Antifertility, methanolic extract, sperm count, morphological characteristics, sperm motility, Azadirachta indica.

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[1]
Ain, Q.U.; David, M.; Shah, Q.; Ahmad, M.; Jahan, S. Antifertility effect of methanolic leaf extract of Chenopodium ambrosioides hook in male Sprague Dawley rats. Andrologia, 2018, 50(10), e13129.
[http://dx.doi.org/10.1111/and.13129] [PMID: 30125378]
[2]
Worldometers. Real time World Statistics., http://www.worldometers.info/es/ [Jan, 31, 2019]; 2019
[3]
Heinemann, K.; Saad, F.; Wiesemes, M.; White, S.; Heinemann, L. Attitudes toward male fertility control: Results of a multinational survey on four continents. Hum. Reprod., 2005, 20(2), 549-556.
[http://dx.doi.org/10.1093/humrep/deh574] [PMID: 15608042]
[4]
Plana, O. Male contraception: Research, new methods, and implications for marginalized populations. Am. J. Men Health, 2017, 11(4), 1182-1189.
[http://dx.doi.org/10.1177/1557988315596361] [PMID: 26206159]
[5]
Serfaty, D. Update on the contraceptive contraindications. J. Gynecol. Obstet. Hum. Reprod., 2019, 48(5), 297-307.
[http://dx.doi.org/10.1016/j.jogoh.2019.02.006] [PMID: 30796985]
[6]
Matzuk, M.M.; McKeown, M.R.; Filippakopoulos, P.; Li, Q.; Ma, L.; Agno, J.E.; Lemieux, M.E.; Picaud, S.; Yu, R.N.; Qi, J.; Knapp, S.; Bradner, J.E. Small-molecule inhibition of BRDT for male contraception. Cell, 2012, 150(4), 673-684.
[http://dx.doi.org/10.1016/j.cell.2012.06.045] [PMID: 22901802]
[7]
Verma, H.P.; Singh, S.K. Antifertility efficacy of Coccinia indica in male mice and its possible mechanisms of action on spermatogenesis. Gen. Comp. Endocrinol., 2017, 241(241), 89-99.
[http://dx.doi.org/10.1016/j.ygcen.2016.05.007] [PMID: 27174748]
[8]
Oluyemi, K.A.; Omotuyi, I.O.; Jimoh, O.R.; Adesanya, O.A.; Saalu, C.L.; Josiah, S.J. Erythropoietic and anti-obesity effects of Garcinia cambogia (bitter kola) in Wistar rats. Biotechnol. Appl. Biochem., 2007, 46(Pt 1), 69-72.
[http://dx.doi.org/10.1042/BA20060105] [PMID: 16984227]
[9]
Ghosh, A.; Pakhira, B.P.; Tripathy, A.; Ghosh, D. Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat. Pharm. Biol., 2017, 55(1), 2035-2042.
[http://dx.doi.org/10.1080/13880209.2017.1357734 ] [PMID: 28836479]
[10]
Moga, M.; Bălan, A.; Anastasiu, C.; Dimienescu, O.; Neculoiu, C.; Gavri, C. An overview on the anticancer activity of Azadirachta indica (Neem) in gynecological cancers. Int. J. Mol. Sci., 2018, 1919(12), 3898.
[http://dx.doi.org/10.3390/ijms19123898]
[11]
Drabu, S.; Khatri, S.; Babu, S. Neem: Healer of all ailments. Res. J. Pharm. Biol. Chem. Sci., 2012, 3, 120-126.
[12]
Arévalo-Híjar, L.; Aguilar-Luis, M.Á.; Caballero-García, S.; Gonzáles-Soto, N.; Del Valle-Mendoza, J. Antibacterial and cytotoxic effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) methanolic extracts against strains of Enterococcus faecalis. Int. J. Dent., 2018, 2018, 1071676.
[http://dx.doi.org/10.1155/2018/1071676] [PMID: 30356384]
[13]
Blum, F.C.; Singh, J.; Merrell, D.S. In vitro activity of neem (Azadirachta indica) oil extract against Helicobacter pylori. J. Ethnopharmacol., 2019, 232, 236-243.
[http://dx.doi.org/10.1016/j.jep.2018.12.025] [PMID: 30578933]
[14]
WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants; World Health Organization: Geneva, 2003.
[15]
López, L.; García, H. Antioxidant activity of methanolic and aqueous extracts of different varieties of mexican maize). Nova Scientia, 2009, 2-1(3), 51-65.
[16]
McLaughlin, J.L. Paw paw and cancer: Annonaceous acetogenins from discovery to commercial products. J. Nat. Prod., 2008, 71(7), 1311-1321.
[http://dx.doi.org/10.1021/np800191t] [PMID: 18598079]
[17]
Sabai, K.; Kwa, N.; Kyi, S.; Tin, M.H. Evaluation of the bioactivities of some Myanmar medicinal plants using brine shrimp (Artemia salina) toxicity test. J. Myan. Acad. Tech., 2001, 1, 105-112.
[18]
Lewan, L.; Andersson, M.; Morales-Gómez, P. The use of Artemia salina in toxicity. Testing Alternat. Lab. Anim., 1992, 20, 297-301.
[19]
Bezerra, D.P.; Pessoa, C.; de Moraes, M.O.; Silveira, E.R.; Lima, M.A.; Elmiro, F.J.; Costa-Lotufo, L.V. Antiproliferative effects of two amides, piperine and piplartine, from Piper species. Z. Natforsch. C J. Biosci., 2005, 60(7-8), 539-543.
[http://dx.doi.org/10.1515/znc-2005-7-805] [PMID: 16163826]
[20]
Logarto Parra, A.; Silva Yhebra, R.; Guerra Sardiñas, I.; Iglesias Buela, L. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine, 2001, 8(5), 395-400.
[http://dx.doi.org/10.1078/0944-7113-00044] [PMID: 11695884]
[21]
Ferreira, P.M.P.; Santos, D.B.; Silva, J.D.N.; Goudinho, A.F.; Ramos, C.L.S.; Souza, P.C.; Almeida, R.S.C.; Moura, D.S.; Oliveira, R.; Grisolia, C.K.; Cavalheiro, A.J.; Carvalho Melo-Cavalcante, A.A.; Ferreira, J.R.O.; Moraes Filho, M.O.; Pessoa, C. Toxicological findings about an anticancer fraction with casearins described by traditional and alternative techniques as support to the Brazilian Unified Health System (SUS). J. Ethnopharmacol., 2019, 241, 112004.
[http://dx.doi.org/10.1016/j.jep.2019.112004] [PMID: 31152784]
[22]
Kuklinski, K.C. Farmacognosia (QUIMICA), 1st ed.; Omega: Libros , 1999.
[23]
Iraqui, P.; Borah, D.; Kardong, D.; Yadav, R. Qualitative and Quantitative screening of phytochemicals of Meliosommma pinnata (dermi) a forest based vegetable plant traditionally used by mising community of Assam, India. Int. J. Pharm. Pharm. Sci., 2013, 5, 200-203.
[24]
Omokhua, A.G.; Abdalla, M.A.; Van Staden, J.; McGaw, L.J. A comprehensive study of the potential phytomedicinal use and toxicity of invasive Tithonia species in South Africa. BMC Complement. Altern. Med., 2018, 18(1), 272.
[http://dx.doi.org/10.1186/s12906-018-2336-0] [PMID: 30285713]
[25]
Ramírez, A.; Serrano, L.; Barragán, L.; Quintanar, M.; Arellano, R.; Delgadillo, D. Determination of polyphenolic compounds in Jatropha dioica extracts and their antioxidant capacity. Rev. Mex. Cienc. Farm., 2016, 47(4)
[26]
Magalhães, L.M.; Santos, F.; Segundo, M.A.; Reis, S.; Lima, J.L. Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity. Talanta, 2010, 83(2), 441-447.
[http://dx.doi.org/10.1016/j.talanta.2010.09.042] [PMID: 21111158]
[27]
World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction, 2001.
[28]
Mwangi, G.G.; Wagacha, J.M.; Nguta, J.M.; Mbaria, J.M. Brine shrimp cytotoxicity and antimalarial activity of plants traditionally used in treatment of malaria in Msambweni district. Pharm. Biol., 2015, 53(4), 588-593.
[http://dx.doi.org/10.3109/13880209.2014.935861] [PMID: 25495507]
[29]
Datta, A.; Grün, I.U.; Kwasniewski, M.T.; Fernando, L.N. Comparison of two adsorbent based de-bittering procedures for neem (Azadirachta indica A. Juss) tea- effect on polyphenols, anti-oxidant capacity, color and volatile profile. Plant Foods Hum. Nutr., 2017, 72(1), 88-95.
[http://dx.doi.org/10.1007/s11130-016-0595-9] [PMID: 28091970]
[30]
Mukherjee, N.; Mukherjee, S.; Saini, P.; Roy, P.; Sinha Babu, S.P. Antifilarial effects of polyphenol rich ethanolic extract from the leaves of Azadirachta indica through molecular and biochemical approaches describing Reactive Oxygen Species (ROS) mediated apoptosis of Setaria cervi. Exp. Parasitol., 2014, 136, 41-58.
[http://dx.doi.org/10.1016/j.exppara.2013.11.006] [PMID: 24275557]
[31]
Shehab, N.G.; Abu-Gharbieh, E. Phenolic profiling and evaluation of contraceptive effect of the ethanolic extract of Salsola imbricata Forssk in male albino rats. Evid. Based Complement. Alternat. Med., 2014, 2014, 695291.
[http://dx.doi.org/10.1155/2014/695291] [PMID: 25587346]
[32]
Ahangarpour, A.; Oroojan, A.A.; Radan, M. Effect of aqueous and hydro-alcoholic extracts of lettuce (Lactuca sativa) seed on testosterone level and spermatogenesis in NMRI mice. Iran. J. Reprod. Med., 2014, 12(1), 65-72.
[PMID: 24799863]
[33]
Mishra, N.; Joshi, S.; Tandon, V.; Munjal, A. Evaluation of anti-fertility potential of aqueous extract of Bougainvillea spectabilis leaves in swiss albino mice. Int. J. Pharm. Sci. Drug Res., 2009, 1(1), 19-23.
[34]
Jain, S.; Jain, A.; Paliwal, P.; Solanki, S.S. Antifertility effect of chronically administered Tabernaemontana divaricata leaf extract on male rats. Asian Pac. J. Trop. Med., 2012, 5(7), 547-551.
[http://dx.doi.org/10.1016/S1995-7645(12)60096-0 ] [PMID: 22647817]
[35]
Khillare, B.; Shrivastav, T.G. Spermicidal activity of Azadirachta indica (neem) leaf extract. Contraception, 2003, 68(3), 225-229.
[http://dx.doi.org/10.1016/S0010-7824(03)00165-3 ] [PMID: 14561544]
[36]
D’Cruz, S.C.; Vaithinathan, S.; Jubendradass, R.; Mathur, P.P. Effects of plants and plant products on the testis. Asian J. Androl., 2010, 12(4), 468-479.
[http://dx.doi.org/10.1038/aja.2010.43] [PMID: 20562897]
[37]
Purohit, A. Antifertility efficacy of Neem Bark (Azadirachta indica A. juss.) in male rats. Anc. Sci. Life, 1999, 19(1-2), 21-24.
[PMID: 22556912]

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