Abstract
Background: Plant derived compounds, naringenin and ellagic acid might interfere in MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay in the form of reducing the tetrazolium salt in the absence of cells.
Objective: Effect of naringenin and ellagic acid in MTT assay.
Methods: MTT assay has been performed in different conditions like, varying the concentration of phytocompounds, incubation time, and changing the media.
Results: Ellagic acid at the concentration of 80 μg/ml showed maximum absorbance of 0.579 in the presence of RPMI media (Roswell Park Memorial Institute Medium) that has 10% newborn calf serum. Naringenin showed higher absorbance of 2.74 at 80 μg/ml in RPMI media that has 10% newborn calf serum. Naringenin and Ellagic acid had better ability to reduce the MTT in the presence of RPMI media than anhydrous ethanol.
Conclusion: Naringenin reduced the MTT better than ellagic acid in RPMI-1640 medium. These results show the need to be cautious when choosing MTT assay to measure the metabolic activity of cells treated with phytocompounds.
Keywords: Naringenin, ellagic acid, MTT, absorbance, absence of cells, phytocompounds.
The Natural Products Journal
Title:Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells
Volume: 11 Issue: 4
Author(s): Vaibhav Tiwary, Priyanka Sarkar and Kavitha Thirumurugan*
Affiliation:
- Structural Biology Lab, Center for Bio Medical Research, School of Bio Sciences Technology, Vellore Institue of Technology, Tamil Nadu,India
Keywords: Naringenin, ellagic acid, MTT, absorbance, absence of cells, phytocompounds.
Abstract:
Background: Plant derived compounds, naringenin and ellagic acid might interfere in MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay in the form of reducing the tetrazolium salt in the absence of cells.
Objective: Effect of naringenin and ellagic acid in MTT assay.
Methods: MTT assay has been performed in different conditions like, varying the concentration of phytocompounds, incubation time, and changing the media.
Results: Ellagic acid at the concentration of 80 μg/ml showed maximum absorbance of 0.579 in the presence of RPMI media (Roswell Park Memorial Institute Medium) that has 10% newborn calf serum. Naringenin showed higher absorbance of 2.74 at 80 μg/ml in RPMI media that has 10% newborn calf serum. Naringenin and Ellagic acid had better ability to reduce the MTT in the presence of RPMI media than anhydrous ethanol.
Conclusion: Naringenin reduced the MTT better than ellagic acid in RPMI-1640 medium. These results show the need to be cautious when choosing MTT assay to measure the metabolic activity of cells treated with phytocompounds.
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Cite this article as:
Tiwary Vaibhav , Sarkar Priyanka and Thirumurugan Kavitha *, Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells, The Natural Products Journal 2021; 11 (4) . https://dx.doi.org/10.2174/2210315510999200723162422
DOI https://dx.doi.org/10.2174/2210315510999200723162422 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |
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