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Current Genomics

Editor-in-Chief

ISSN (Print): 1389-2029
ISSN (Online): 1875-5488

Letter Article

Discrepant mRNA and Protein Expression in Immune Cells

Author(s): Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong*

Volume 21, Issue 8, 2020

Page: [560 - 563] Pages: 4

DOI: 10.2174/1389202921999200716103758

Price: $65

Abstract

With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.

Keywords: Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.

Graphical Abstract
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