Title:Altered Expression of microRNAs in Serum Extracellular Vesicles in Rats with Severe Burns during Shock Stage
VOLUME: 20
Author(s):Mengru Dong, Yanbin Gao, Minxiong Li and Lei Yang*
Affiliation:Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515
Keywords:Extracellular vesicles, miRNA, severe burns, Shock stage, Functional enrichment analysis.
Abstract:Background: The shock stage of severe burns is a critical determinant of prognosis and the induction of systemic
inflammatory response syndrome and multiple organ failure. Extracellular vesicles (EVs) containing abundant miRNAs are
known to participate in various biological processes. Due to lack of researches in alternations of miRNAs in severe burns,
our study analyzed the miRNA profiles of EVs in severe burns during shock stage.
Methods: EVs were extracted from serum of rats with severe burns (30% of total body surface area, III°), and the expression
of miRNAs in serum EVs was determined by next generation sequencing. Functional analysis of target genes of miRNAs
that were significantly differentially expressed (DE) was performed using GO Ontology (GO) and Kyoto Encyclopedia of
Genes and Genomes (KEGG).
Results: Thirty-four DE miRNAs were identified at the early stage of severe burn shock and 63 at the late stage of severe
burn shock. In addition, miRNA-339-5p, miRNA-1, miRNA-382-5p, miRNA134-5p, miRNA-133a-5p, and miRNA-365a5p were DE throughout the entire shock stage, based on P < 0.01 and |log2 (foldchange)| ≥ 1 criteria. GO and KEGG analysis revealed that the target genes of DE miRNAs mainly enriched metabolic process, immune system processes, and signal
pathways.
Conclusion: To our best knowledge, this is the first study demonstrating the miRNA expression profiles of EVs isolated
from serum with severe burns during shock stage. There are significant differences in downregulation and upregulation.
Thus, miRNAs have potential for novel biomarkers for the complication of severe burns.
