Background and objectives: The relationship between vascular damage and diabetes mellitus was exploited
using avocado seed extracts. The purpose of the study was to understand the therapeutic relevance of
glycosides compared to standard vascular and anti-diabetic drugs. Constituent avocado seed glycosides
(ASG) were analysed and administered to rats with diabetes-induced vascular damage (DIVD).
Method: The rats were first administered with streptozotocin and screened after seven days for alterations
in blood glucose, insulin, vascular cell adhersion molecule (VCAM-1), Von Willebrond factor (VWF),
Renin-Angiotensin-Aldosterone System(RAS), eNOx, and endothelin-1 (ET-1). Only rats that satisfied
these criteria were recruited and treated with either glibenclamide, met.su + losart, or 200 mg/kg body
weight ASG for 28 days.
Result: There was an abundance of digitoxin (13.41 mg/100g), digoxin (17.98 mg/100g), avicularin (165.85
mg/100g), and hyperoside (282.51 mg/100g). ASG or met.su + losartexhibited slight modulatory properties
on glucose homeostasis. Rats withDIVD showed elevated renin, angiotensin, VCAM-1 and Lp-PLA2 levels
but slightly decreased with glibenclamide treatment and normalized with ASG or met.su + losart
administration. All treatments normalized Hcy levels. DIVD caused the overproduction of CnT, LDH, CrtK, LDL-c, TG, and TC and suppressed HDL-c but were completely normalized by the ASG. Water intake
remained altered in treated rats.
Conclusion: The ASG had no relevant effect on glucose homeostasis during DIVD but showed significant