Background: Hepatocellular carcinoma (HCC) is a prevalent cancer in the world. As yet, there is no medication for
complete treatment of HCC. Objective: There is a critical need to search for an innovative therapy for HCC. Recently,
multi-epitope vaccines have introduced as an effective immunotherapy approach against HCC.
Methods: In this
research, several immunoinformatics methods were applied to create an original multi-epitope vaccine against HCC
consisting of CD8+ cytolytic T lymphocytes (CTLs) epitopes selected from α- fetoprotein (AFP), glypican-3 (GPC3),
aspartyl-β-hydroxylase (ASPH); CD4+ helper T lymphocytes (HTLs) epitopes from tetanus toxin fragment C (TTFC),
and finally, two tandem repeats of HSP70407-426 were which stimulate strong innate and adaptive immune responses.
All the mentioned parts were connected together by relevant linkers.
Results: According to physicochemical,
structural, and immunological results, the designed vaccine is stable, non-allergen, antigen; it also has a high-quality
3D structure, and numerous linear and conformational B cell epitopes, whereby this vaccine may stimulate efficient
Conclusion: Center on the collected results, the designed vaccine potentially can induce cellular
and humoral immune responses in HCC cases; nonetheless, the efficiency of vaccine must be approved within in
vitro and in vivo immunological analyzes.