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Anti-Cancer Agents in Medicinal Chemistry

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ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

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Research Article

Isolation, Characterization and Preliminary Cytotoxic and Antifungal Evaluations of Novel Lancifoliate Isolated from Methanol Extract of Conocarpus lancifolius

Author(s): Malik Saadullah*, Muhammad Asif, Bashir A. Ch, Hafiza S. Yaseen, Muhammad Uzair and Khurram Afzal

Volume 20, Issue 14, 2020

Page: [1664 - 1672] Pages: 9

DOI: 10.2174/1871520620666200424110923

Price: $65

Abstract

Background: Combretaceae is a large family comprising of 500 species and 20 genera distributed in subtropical and tropical regions of the world. Conocarpus genus is an ornamental tree native to coastal and riverine areas of East Africa and is also planted as an ornamental plant in different areas of Pakistan. This genus has proved medicinal value as a cytotoxic, antibacterial, antiprotozoal, anti-leishmanial, antifungal and antidiabetic agent.

Objective: The current study was designed to screen the selected pharmacological attributes of sulphur containing novel compound isolated from Conocarpus lancifolius using a series of in vitro and molecular docking models.

Materials and Methods: After collection and authentication of plant material, methanolic extract was prepared from which various secondary metabolites were qualitatively examined. The compound was isolated using open column chromatography and the structure was established with spectroscopic techniques such as UV-visible, infrared spectroscopy, proton nuclear magnetic resonance (1H-NMR), 13C NMR (BB, DEPT-135, 90), twodimensional correlation techniques (HMBC, HSQC) and mass spectrometry (HRMS) respectively. C. lancifolius extract and isolated compound were studied for cytotoxic and antifungal potentials using in vitro Sulforhodamine B (SRB) and disc diffusion methods, respectively. Molecular docking studies were conducted to check the interaction of the isolated compound with major oncogenic proteins.

Results: Qualitative phytochemical screening revealed the presence of saponins, steroids, flavonoids, anthraquinones, and cardiac glycosides while alkaloids were absent in C. lancifolius extract. Isolated compound was characterized as lancifoliate, which showed cytotoxic activity towards a variety of cancer cell lines including murine lymphocytic leukemia (P-388, IC50 = 2.65μg/ml), human colon cancer (Col-2, IC50 = 0.84μg/ml), human breast cancer (MCF-7, IC50 = 0.72μg/ml) while no cytotoxic activity was observed towards human lung cancer (Lu-1), rat normal glioma cells (ASK, IC50 = 11.6μg/ml) and human embryonic kidney cells (Kek293, IC50 = 6.74μg/ml) respectively. Minimum Inhibitory Concentration (MIC) of Lancifoliate towards Aspergillus fumigatus, Aspergillus nigar (skin sample), Aspergillus flavus (pleural fluid) and Candida albicans (urine and blood samples) was found to be 54.5, 44.8, 43.5, 22.4 and 20.2μg/ml respectively. Moreover, docking results are in strong agreement with our experimental finding, which has identified lancifoliate to be a more potent antiproliferative agent than previously known compound ellipticine.

Conclusion: C. lancifolius extract and lancifoliate possess potent cytotoxic and antifungal properties and thus has potential to be further studied. To the best of our knowledge, this is the first study that highlights isolation, identification and pharmacological activities of lancifoliate from Conocarpus lancifolius.

Keywords: Conocarpus lancifolius, Combretaceae, cytotoxic activity, antifungal, molecular docking, methanol extract.

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[1]
Siegel, R.L.; Miller, K.D.; Jemal, A. Cancer statistics, 2016. CA Cancer J. Clin., 2016, 66(1), 7-30.
[http://dx.doi.org/10.3322/caac.21332] [PMID: 26742998]
[2]
Greenstein, J.P. Biochemistry of Cancer; Elsevier, 2016.
[3]
Vineis, P.; Wild, C.P. Global cancer patterns: Causes and prevention. Lancet, 2014, 383(9916), 549-557.
[http://dx.doi.org/10.1016/S0140-6736(13)62224-2] [PMID: 24351322]
[4]
Cassiani, S.; Von Linsingen, I. Formação inicial de professores de Ciências: Perspectiva discursiva na educação CTS. Educar em Revista, 2009, 34.
[http://dx.doi.org/10.1590/S0104-40602009000200008]
[5]
Abdel-Hameed, E-S.S. Phytochemical studies and evaluation of antioxidant, anticancer and antimicrobial properties of Conocarpus erectus L. growing in Taif, Saudi Arabia. Eur. J. Med. Plants, 2012, 2(2), 93.
[http://dx.doi.org/10.9734/EJMP/2012/1040]
[6]
Baroon, Z.; Razzaque, M.A. Nutritional evaluation and palatability trial of ensiled conocarpus greenery residues. Exp. Agric., 2011, 48(1), 138-147.
[http://dx.doi.org/10.1017/S0014479711000871]
[7]
Redha, A.M.; Dincer, I.; Gadalla, M. Thermodynamic performance assessment of wind energy systems. Appl. Energy, 2011, 36(7), 4002-4010.
[http://dx.doi.org/10.1016/j.energy.2011.05.001]
[8]
Rasheed, F. Phytoaccumulation of Zn, Pb, and Cd in Conocarpus lancifolius irrigated with wastewater: does physiological response influence heavy metal uptake? Int. J. Phytoremediation, 2019, 22(3), 287-294.
[PMID: 31468990]
[9]
Abdel-Megeed, A. In vitro antibacterial activities of alkaloids extract from leaves of Conocarpus lancifolius. Engl. J. Pure Appl. Microbiol., 2013, 7, 1903-1907.
[10]
Saleem, M.; Javed, F.; Asif, M.; Baig, M.K.; Arif, M. HPLC analysis and in vivo renoprotective evaluation of hydroalcoholic extract of Cucumis melo seeds in gentamicin-induced renal damage. Medicina (Kaunas), 2019, 55(4) E107
[http://dx.doi.org/10.3390/medicina55040107] [PMID: 30991760]
[11]
Vichai, V.; Kirtikara, K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat. Protoc., 2006, 1(3), 1112-1116.
[http://dx.doi.org/10.1038/nprot.2006.179] [PMID: 17406391]
[12]
Hati, S.; Tripathy, S.; Dutta, P.K.; Agarwal, R.; Srinivasan, R.; Singh, A.; Singh, S.; Sen, S. Spiro[pyrrolidine-3, 3´-oxindole] as potent anti-breast cancer compounds: Their design, synthesis, biological evaluation and cellular target identification. Sci. Rep., 2016, 6(1), 32213.
[http://dx.doi.org/10.1038/srep32213] [PMID: 27573798]
[13]
Balouiri, M.; Sadiki, M.; Ibnsouda, S.K. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 2016, 6(2), 71-79.
[http://dx.doi.org/10.1016/j.jpha.2015.11.005] [PMID: 29403965]
[14]
Majumdar, P.; Bathula, C.; Basu, S.M.; Das, S.K.; Agarwal, R.; Hati, S.; Singh, A.; Sen, S.; Das, B.B. Design, synthesis and evaluation of thiohydantoin derivatives as potent topoisomerase I (Top1) inhibitors with anticancer activity. Eur. J. Med. Chem., 2015, 102, 540-551.
[http://dx.doi.org/10.1016/j.ejmech.2015.08.032] [PMID: 26312433]
[15]
Olano, C.; Méndez, C.; Salas, J.A. Antitumor compounds from marine actinomycetes. Mar. Drugs, 2009, 7(2), 210-248.
[http://dx.doi.org/10.3390/md7020210] [PMID: 19597582]
[16]
Greenwell, M.; Rahman, P.K. Medicinal plants: Their use in anticancer treatment. Int. J. Pharm. Sci. Res., 2015, 6(10), 4103-4112.
[PMID: 26594645]
[17]
Prota, A.E.; Bargsten, K.; Zurwerra, D.; Field, J.J.; Díaz, J.F.; Altmann, K.H.; Steinmetz, M.O. Molecular mechanism of action of microtubule-stabilizing anticancer agents. Science, 2013, 339(6119), 587-590.
[http://dx.doi.org/10.1126/science.1230582] [PMID: 23287720]
[18]
Malik, K. Human development report 2014: Sustaining human progress: Reducing vulnerabilities and building resilience; United Nations Development Programme: New York, 2014.
[19]
Cerella, C.; Dicato, M.; Jacob, C.; Diederich, M. Chemical properties and mechanisms determining the anti-cancer action of garlic-derived organic sulfur compounds. Anticancer. Agents Med. Chem., 2011, 11(3), 267-271.
[http://dx.doi.org/10.2174/187152011795347522] [PMID: 21269260]
[20]
Mousset, S.; Buchheidt, D.; Heinz, W.; Ruhnke, M.; Cornely, O.A.; Egerer, G.; Krüger, W.; Link, H.; Neumann, S.; Ostermann, H.; Panse, J.; Penack, O.; Rieger, C.; Schmidt-Hieber, M.; Silling, G.; Südhoff, T.; Ullmann, A.J.; Wolf, H.H.; Maschmeyer, G.; Böhme, A. Treatment of invasive fungal infections in cancer patients-updated recommendations of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann. Hematol., 2014, 93(1), 13-32.
[http://dx.doi.org/10.1007/s00277-013-1867-1] [PMID: 24026426]
[21]
Ramirez-Garcia, A.; Rementeria, A.; Aguirre-Urizar, J.M.; Moragues, M.D.; Antoran, A.; Pellon, A.; Abad-Diaz-de-Cerio, A.; Hernando, F.L. Candida albicans and cancer: Can this yeast induce cancer development or progression? Crit. Rev. Microbiol., 2016, 42(2), 181-193.
[PMID: 24963692]

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