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ISSN (Print): 2212-7984
ISSN (Online): 1876-1429

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Research Article

Bioprocess Optimization for Exopolysaccharides Production by Ganoderma lucidum in Semi-industrial Scale

Author(s): Ramzy Abd Alsaheb, Koh Zwin Zjeh, Roslinda Abd Malek, Jaafar Kamil Abdullah, Ashraf El Baz, Nehal El Deeb, Daniel Dailin, Siti Zulaiha Hanapi, Dalia Sukmawati and Hesham El Enshasy*

Volume 11, Issue 3, 2020

Page: [211 - 218] Pages: 8

DOI: 10.2174/2212798411666200316153148

Abstract

Background: For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid during the last years for the production of mushrooms bioactive compounds in a closed cultivation system to shorten the cultivation time and increase the product yield.

Objectives: This work focuses on the development of a simple cultivation strategy for exopolysaccharides (EPS) production using Ganoderma lucidum and submerged cultivation system.

Methods: At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production processes, carbon and nitrogen concentrations were optimized to support the highest production of polysaccharides in the shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor.

Results: The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH conditions (pH 5.5). Under this condition, the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively.

Conclusion: The current results clearly demonstrate the high potential use of submerged cultivation system as an alternative to conventional solid-state fermentation for EPS production by G. lucidum. Furthermore, the optimization of both carbon and nitrogen sources concentration and scaling up of the process showed a significant increase in both volumetric and specific EPS production.

Keywords: Ganoderma lucidum, submerged culture, Pilot-scale, polysaccharide, mushrooms, morphology.

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[1]
El Enshasy H, Elsayed EA, Aziz R, Wadaan MA. Mushrooms and truffles: historical biofactories for complementary medicine in Africa and in the Middle East. Evid Based Complement Alternat Med 2013.Article ID 620451
[http://dx.doi.org/10.1155/2013/620451]
[2]
Maftoun P, Malek R, Abdel-Sadek M, Aziz R, El Enshasy H. Bioprocess for semi-industrial production of immunomodulator polysaccharide Pleuran by Pleurotus ostreatus in submerged culture. J Sci Ind Res 2013; 72: 655-62.
[3]
El Enshasy H. The Mycota 2010; 165-94.
[4]
El Enshasy HA, Hatti-Kaul R. Mushroom immunomodulators: unique molecules with unlimited applications. Trends Biotechnol 2013; 31(12): 668-77.
[http://dx.doi.org/10.1016/j.tibtech.2013.09.003] [PMID: 24125745]
[5]
Elsayed EA, El Enshasy H, Wadaan MA, Aziz R. Mushrooms: a potential natural source of anti-inflammatory compounds for medical applications. Mediators Inflamm 2014; 2014Article ID 805841
[http://dx.doi.org/10.1155/2014/805841] [PMID: 25505823]
[6]
Masri J, Maftoun P, Abd Malek R, et al. The edible mushroom Pleurotus spp.: II. Medicinal values. Int J Biotechnol Wellness Ind 2017; 6(1): 1-11.
[http://dx.doi.org/10.6000/1927-3037.2017.06.01.1]
[7]
Selvamani S, El-Enshasy HA, Dailin DJ, et al. Antioxidant compounds of the edible mushroom Pleurotus ostreatus. Int J Biotechnol Wellness Ind 2018; 7: 1-14.
[http://dx.doi.org/10.6000/1927-3037.2018.07.01]
[8]
You B-J, Lee H-Z, Chung K-R, et al. Enhanced production of ganoderic acids and cytotoxicity of Ganoderma lucidum using solid-medium culture. Biosci Biotechnol Biochem 2012; 76(8): 1529-34.
[http://dx.doi.org/10.1271/bbb.120270] [PMID: 22878212]
[9]
Ferreira ICFR, Heleno SA, Reis FS, et al. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities. Phytochemistry 2015; 114: 38-55.
[http://dx.doi.org/10.1016/j.phytochem.2014.10.011] [PMID: 25457487]
[10]
Baby S, Johnson AJ, Govindan B. Secondary metabolites from Ganoderma. Phytochemistry 2015; 114: 66-101.
[http://dx.doi.org/10.1016/j.phytochem.2015.03.010] [PMID: 25975187]
[11]
Eroğlu HA, Beytut E. Effect of Ganoderma lucidum polysaccharides on oxidative damage in liver of STZ-diabetic rats. Biomed Res 2018; 29(18): 3436-43.
[12]
El Enshasy H, Daba A, El Demellawy M, Ibrahim A, El Sayed S, El Badry I. Bioprocess development for large scale production of anticancer exo-polysaccharide by Pleurotus ostreatus in submerged culture. J Appl Sci 2010; 10: 2523-9.
[http://dx.doi.org/10.3923/jas.2010.2523.2529]
[13]
El Enshasy HA, Maftoun P, Abd Malek R. Pleuran. Immunomodulotor Polysaccharide from Pleurotus ostreatus, Structure, Production and Application. In: Andres S, Baumann N. Eds. Mushrooms: Types, Properties and Nutrition. New York: Nova publisher 2012: 153-72..
[14]
Soltani M, Abd Malek R, Ware I, et al. Optimization of cordycepin extraction from Cordyceps militaris fermentation broth. J Sci Ind Res 2017; 76: 355-61.
[15]
Chang MY, Ysai GJ, Houng JY. Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enzyme Microb Technol 2006; 38: 407-14.
[http://dx.doi.org/10.1016/j.enzmictec.2005.06.011]
[16]
Hsieh C, Tseng MH, Liu CJ. Production of polysaccharides from Ganoderma lucidum (CCR 36041) under limitation of nutrients. Enzyme Microb Technol 2006; 38: 109-17.
[http://dx.doi.org/10.1016/j.enzmictec.2005.05.004]
[17]
Zhang JG, Chen XM. He XS. A study of synthetic characteristics of the extracellular polysaccharide (EPS) of Ganoderma lucidum cultured in batch fermentation using a kinetic model. Chin J Biotechnol 2007; 23: 1065-71.
[http://dx.doi.org/10.1016/S1872-2075(07)60064-8]
[18]
Lee KM, Lee SY, Lee HY. Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. J Biosci Bioeng 1999; 88(6): 646-50.
[http://dx.doi.org/10.1016/S1389-1723(00)87094-2] [PMID: 16232678]
[19]
Yang HL, Wu TX, Zhang KC. Enhancement of mycelial growth and polysaccharide production in Ganoderma lucidum (the Chinese medicinal fungus, ‘Lingzhi’) by the addition of ethanol. Biotechnol Lett 2004; 26(10): 841-4.
[http://dx.doi.org/10.1023/B:BILE.0000025888.93564.a0] [PMID: 15269558]
[20]
Bae JT, Sinha J, Park JP, Song CH, Yun JW. Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J Microbiol Biotechnol 2000; 10: 482-7.
[21]
El-Enshasy HA, Mohamed NA, Farid MA, El-Diwany AI. Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization. Bioresour Technol 2008; 99(10): 4263-8.
[http://dx.doi.org/10.1016/j.biortech.2007.08.050] [PMID: 17936622]
[22]
Awad HM, El-Shahed KYI, Aziz RA, Sarmidi MR, El Enshasy HA. Antibiotics as microbial secondary metabolites: production and application. Jurnal Tekologi 2012; 59: 101-11.
[23]
Esawy MA, Amer H, Gamal-Eldeen AM, et al. Scaling up, characterization of levan and its inhibitory role in carcinogenesis initiation stage. Carbohydr Polym 2013; 95(1): 578-87.
[http://dx.doi.org/10.1016/j.carbpol.2013.02.079] [PMID: 23618309]
[24]
Ragab TIM, Malek RA, Elsehemy IA, et al. Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives. J Biosci Bioeng 2019; 127(6): 655-62.
[http://dx.doi.org/10.1016/j.jbiosc.2018.09.008] [PMID: 30795878]
[25]
Then C, Wai OK, Elsayed EA, et al. Comparison between classical and statistical medium optimization approaches for high cell mass production of Azotobacter vinelandii. J Sci Ind Res 2016; 75: 231-8.
[26]
El Enshasy H, Then C, Othman NZ, et al. Enhanced xanthan production process in shake flasks and pilot scale bioreactors using industrial semi-defined medium. Afr J Biotechnol 2011; 10: 1029-38.
[27]
Then C, Othman Z, Wan Mustapha WA, Sarmidi MR, Aziz R, El Enshasy HA. Production of alginate by Azotobacter vinelandii in semi-industrial scale using batch and fed-batch cultivation systems. Adv J Sci Res 2012; 3: 45-50.

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