Evaluation of Thiol/Disulfide Homeostasis in Pediatric Patients with Diabetic Ketoacidosis

Author(s): Mutlu U. Yazıcı*, Ganime Ayar, Semra Çetinkaya, Meliksah Keskin, Ebru Azapağası, Saim Neşelioğlu, Özcan Erel, Benan Bayrakçı

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 23 , Issue 3 , 2020


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Abstract:

Aims and Objective: This study aimed to investigate the value of Thiol/Disulfide homeostasis in pediatric diabetic ketoacidosis patients suffering from type 1 diabetes mellitus.

Materials and Methods: This study featured children who were diagnosed with diabetic ketoacidosis and who were consecutively admitted to pediatric intensive care within one year of their diagnosis. Thiol/disulfide homeostasis was evaluated in 45 pediatric patients suffering from DKA, as well as 45 healthy controls of parallel gender and age. Thiol/disulfide homeostasis parameters were measured using a novel automated measurement method and the correlation between demographic data and parameters was measured.

Results: Pediatric patients were found to have low native thiols, total thiols and disulfide levels with type 1 diabetes after DKA (331.82±106.40, 362.71±113.31, 17.02±5.33 μmol/L, respectively) as compared to the control group (445.08±24.41, 481.21± 28.47, 18.06±5.12 μmol/L, respectively).

Conclusion: Thiol/disulfide homeostasis was distorted in pediatric patients with DKA. Furthermore, it was found that they are not likely to return to normal, immediately after treatment.

Keywords: Thiol/disulfide homeostasis, diabetic ketoacidosis, type 1 diabetes mellitus, children, hyperglycemia, hyperketonemia.

[1]
van Belle, T.L.; Coppieters, K.T.; von Herrath, M.G. Type 1 diabetes: etiology, immunology, and therapeutic strategies. Physiol. Rev., 2011, 91(1), 79-118.
[http://dx.doi.org/10.1152/physrev.00003.2010] [PMID: 21248163]
[2]
IDF Diabetes Atlas – 8th Edition. Available at:. https://www.idf.org/sites (Accessed on 20 Apr 2019).
[3]
Dunger, D.B.; Sperling, M.A.; Acerini, C.L.; Bohn, D.J.; Daneman, D.; Danne, T.P.; Glaser, N.S.; Hanas, R.; Hintz, R.L.; Levitsky, L.L.; Savage, M.O.; Tasker, R.C.; Wolfsdorf, J.I. ESPE/LWPES consensus statement on diabetic ketoacidosis in children and adolescents. Arch. Dis. Child., 2004, 89(2), 188-194.
[http://dx.doi.org/10.1136/adc.2003.044875] [PMID: 14736641]
[4]
Jain, S.K.; McVie, R.; Bocchini, J.A., Jr Hyperketonemia (ketosis), oxidative stress and type 1 diabetes. Pathophysiology, 2006, 13(3), 163-170.
[http://dx.doi.org/10.1016/j.pathophys.2006.05.005] [PMID: 16782314]
[5]
Patel, V.; Rassam, S.M.B.; Chen, H.C.; Kohner, E.M. Oxygen reactivity in diabetes mellitus: effect of hypertension and hyperglycaemia. Clin. Sci. (Lond.), 1994, 86(6), 689-695.
[http://dx.doi.org/10.1042/cs0860689] [PMID: 8062505]
[6]
Ceriello, A. Acute hyperglycaemia and oxidative stress generation. Diabet. Med., 1997, 14(3), S45-S49.http://dx.doi.org/10.1002/(SICI)1096-9136(199708)14:3+<S45:: AID-DIA444>3.3.CO;2-I
[PMID: 9272613]
[7]
Domínguez, C.; Ruiz, E.; Gussinye, M.; Carrascosa, A. Oxidative stress at onset and in early stages of type 1 diabetes in children and adolescents. Diabetes Care, 1998, 21(10), 1736-1742.
[http://dx.doi.org/10.2337/diacare.21.10.1736] [PMID: 9773740]
[8]
Winther, J.R.; Thorpe, C. Quantification of thiols and disulfides. Biochim. Biophys. Acta, 2014, 1840(2), 838-846.
[http://dx.doi.org/10.1016/j.bbagen.2013.03.031] [PMID: 23567800]
[9]
Soysal, P.; Isik, A.T.; Carvalho, A.F.; Fernandes, B.S.; Solmi, M.; Schofield, P.; Veronese, N.; Stubbs, B. Oxidative stress and frailty: A systematic review and synthesis of the best evidence. Maturitas, 2017, 99, 66-72.
[http://dx.doi.org/10.1016/j.maturitas.2017.01.006] [PMID: 28364871]
[10]
Halil, H.; Tuygun, N.; Aksoy, E.; Erel, O.; Karacan, C.D. Serum thiol-disulphide levels in epileptic pediatric patients. Comb. Chem. High Throughput Screen., 2019, 22(1), 65-68.
[http://dx.doi.org/10.2174/1386207322666190305135043] [PMID: 30834829]
[11]
Wolfsdorf, J.I.; Glaser, N.; Agus, M.; Fritsch, M.; Hanas, R.; Rewers, A.; Sperling, M.A.; Codner, E. ISPAD Clinical Practice Consensus Guidelines 2018: Diabetic ketoacidosis and the hyperglycemic hyperosmolar state. Pediatr. Diabetes, 2018, 19(27), 155-177.
[http://dx.doi.org/10.1111/pedi.12701] [PMID: 29900641]
[12]
Erel, O.; Neselioglu, S. A novel and automated assay for thiol/disulphide homeostasis. Clin. Biochem., 2014, 47(18), 326-332.
[http://dx.doi.org/10.1016/j.clinbiochem.2014.09.026] [PMID: 25304913]
[13]
Wolfsdorf, J.I. The International Society of Pediatric and Adolescent Diabetes guidelines for management of diabetic ketoacidosis: Do the guidelines need to be modified? Pediatr. Diabetes, 2014, 15(4), 277-286.
[http://dx.doi.org/10.1111/pedi.12154] [PMID: 24866064]
[14]
Cecilia, O.M.; José Alberto, C.G.; José, N.P.; Ernesto Germán, C.M.; Ana Karen, L.C.; Luis Miguel, R.P.; Ricardo Raúl, R.R.; Adolfo Daniel, R.C. Oxidative stress as the main target in diabetic retinopathy pathophysiology. J. Diabetes Res., 2019, 2019 Article ID 8562408
[http://dx.doi.org/10.1155/2019/8562408] [PMID: 31511825]
[15]
Firoozrai, M.; Nourbakhsh, M.; Razzaghy-Azar, M. Erythrocyte susceptibility to oxidative stress and antioxidant status in patients with type 1 diabetes. Diabetes Res. Clin. Pract., 2007, 77(3), 427-432.
[http://dx.doi.org/10.1016/j.diabres.2007.02.001] [PMID: 17360068]
[16]
Pitocco, D.; Zaccardi, F.; Di Stasio, E.; Romitelli, F.; Santini, S.A.; Zuppi, C.; Ghirlanda, G. Oxidative stress, nitric oxide, and diabetes. Rev. Diabet. Stud., 2010, 7(1), 15-25.
[http://dx.doi.org/10.1900/RDS.2010.7.15] [PMID: 20703435]
[17]
Lin, C.C.; Huang, H.H.; Hu, C.W.; Chen, B.H.; Chong, I.W.; Chao, Y.Y.; Huang, Y.L. Trace elements, oxidative stress and glycemic control in young people with type 1 diabetes mellitus. J. Trace Elem. Med. Biol., 2014, 28(1), 18-22.
[http://dx.doi.org/10.1016/j.jtemb.2013.11.001] [PMID: 24315963]
[18]
Rodrigues, R.; de Medeiros, L.A.; Cunha, L.M.; Garrote-Filho, M.D.S.; Bernardino Neto, M.; Jorge, P.T.; Resende, E.S.; Penha-Silva, N. Correlations of the glycemic variability with oxidative stress and erythrocytes membrane stability in patients with type 1 diabetes under intensive treatment. Diabetes Res. Clin. Pract., 2018, 144, 153-160.
[http://dx.doi.org/10.1016/j.diabres.2018.01.031] [PMID: 29427698]
[19]
Alghobashy, A.A.; Alkholy, U.M.; Talat, M.A.; Abdalmonem, N.; Zaki, A.; Ahmed, I.A.; Mohamed, R.H. Trace elements and oxidative stress in children with type 1 diabetes mellitus. Diabetes Metab. Syndr. Obes., 2018, 11(11), 85-92.
[http://dx.doi.org/10.2147/DMSO.S157348] [PMID: 29618936]
[20]
Dröge, W. Aging-related changes in the thiol/disulfide redox state: implications for the use of thiol antioxidants. Exp. Gerontol., 2002, 37(12), 1333-1345.
[http://dx.doi.org/10.1016/S0531-5565(02)00175-4] [PMID: 12559403]
[21]
Hansen, R.E.; Roth, D.; Winther, J.R. Quantifying the global cellular thiol-disulfide status. Proc. Natl. Acad. Sci. USA, 2009, 106(2), 422-427.
[http://dx.doi.org/10.1073/pnas.0812149106] [PMID: 19122143]
[22]
Vantyghem, M.C.; Balduyck, M.; Zerimech, F.; Martin, A.; Douillard, C.; Bans, S.; Degand, P.M.; Lefebvre, J. Oxidative markers in diabetic ketoacidosis. J. Endocrinol. Invest., 2000, 23(11), 732-736.
[http://dx.doi.org/10.1007/BF03345062] [PMID: 11194706]
[23]
Li, J.; Shen, X. Leptin concentration and oxidative stress in diabetic ketoacidosis. Eur. J. Clin. Invest., 2018, 48(10) e13006
[http://dx.doi.org/10.1111/eci.13006] [PMID: 30053313]
[24]
Vincent, A.M.; McLean, L.L.; Backus, C.; Feldman, E.L. Short-term hyperglycemia produces oxidative damage and apoptosis in neurons. FASEB J., 2005, 19(6), 638-640.
[http://dx.doi.org/10.1096/fj.04-2513fje] [PMID: 15677696]
[25]
Stentz, F.B.; Umpierrez, G.E.; Cuervo, R.; Kitabchi, A.E. Proinflammatory cytokines, markers of cardiovascular risks, oxidative stress, and lipid peroxidation in patients with hyperglycemic crises. Diabetes, 2004, 53(8), 2079-2086.
[http://dx.doi.org/10.2337/diabetes.53.8.2079] [PMID: 15277389]


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Article Details

VOLUME: 23
ISSUE: 3
Year: 2020
Published on: 19 May, 2020
Page: [185 - 190]
Pages: 6
DOI: 10.2174/1386207323666200218114331
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