Preliminary Analysis of Anti-proliferative, Apoptotic, and Anti-migratory Effects llw-3-6 in Skov-3 Ovarian Cystadenocarcinoma Cell Line

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Author(s): Rosalind Gregory-Bass, Leyte L. Winfield*.

Journal Name: Letters in Drug Design & Discovery

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Background: Ovarian cancer and ovarian related diseases affect reproductive health. Therapeutic molecules are needed to improve treatment outcomes and overcome drug resistance. The benzimidazole-based sulphonamide LLW-3-6 has both anti-apoptotic and anti-proliferative effects when used to treat prostate, breast, and brain cancer cells.

Objective: The study described herein evaluates the anti-proliferative and anti-migratory effects of LLW-3-6 SKOV-3 ovarian cystadenocarcinoma cell line.

Methods: Studies were conducted using SKOV-3 cells treated with LLW-3-6. The cell line was propagated and proliferative activity was evaluated by hemocytometric and MTT colorimetric assays. Cellular apoptosis was assessed using Caspase-3 spectrophotometric analysis. Lastly, a scratch wound assay was conducted in a dose and time-dependent manner to assess the effect of LLW-3-6 on migration.

Conclusion: Proliferative studies suggest, SKOV-3 cells exposed to LLW-3-6 in culture resulted in decreased growth and proliferation of cells in a time and dose-dependent manner. The apoptotic effect of this agent was noted with the confirmed presence of Caspase-3 in a dose and timedependent manner as well. Preliminary studies also suggest an anti-migratory effect of LLW-3-6, confirmed by scratch wound analysis. LLW-3-6 is potentially a chemotherapeutic option for decreasing proliferation and inducing apoptosis in ovarian carcinomas. Additional biological analysis is ongoing to further assess the utility of the molecule and its mechanism of action.

Keywords: Anti-migratory, anti-proliferation, benzimidazole, LLW-3-6, ovarian cancer, SKOV-3.

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(E-pub Ahead of Print)
DOI: 10.2174/1570180817666200129142949
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