Title:Ursodeoxycholic Acid (UDCA) Promotes Lactate Metabolism in Mouse Hepatocytes through Cholic Acid (CA) - Farnesoid X Receptor (FXR) Pathway
VOLUME: 20 ISSUE: 8
Author(s):Lu Wang, Huai-Wu He, Xiang Zhou* and Yun Long*
Affiliation:Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing
Keywords:Lactate, ursodeoxycholic acid, cholic acid, farnesoid X receptor, hepatocytes, hyperlactatemia.
Abstract:Background: Persistent hyperlactatemia is associated with greater mortality
in shock. Liver is the main site of lactate metabolism.
Method: In the first part, freshly isolated hepatocytes were incubated in 10% fetal
bovine serum William's E medium supplemented with 10 mM lactate. Cells were then
exposed to 100 μM ursodeoxycholic acid (UDCA), with no addition (control) for 2, 4, 6, 8
h. In the second part, hepatocytes were treated with Silencer select siRNA targeting
FXR or scramble siRNA. The siRNA treatment was repeated twenty four hours later, and
the cells were used in the experiments twenty-four hours after the second treatment.
Then hepatocytes were incubated in 10% fetal bovine serum William's E medium
supplemented with 10 mM lactate. Cells were then exposed to 100 μM UDCA for 2, 4, 6,
8 h. Lactate concentration was determined by ABL80 automatic blood gas analyzer.
Results: UDCA increased ability of hepatocytes to remove lactate. After the knockdown
of FXR, effects caused by UDCA were weakened.
Conclusion: These results demonstrate that UDCA promotes lactate metabolism in
mouse hepatocytes through CA-FXR pathway.
