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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Chemical Composition and Antimicrobial Activity of Essential Oil and Hydrosol Extract From Roots of Plumbago europaea and in-vitro Combinatory Antimicrobial Effect of Hydrosol Extract with Gentamicin and Amphotericin B

Author(s): Faiza Kaddour*, Nadia Aissaoui, Mohammed El Amine Dib, Okkacha Bensaid and Alain Muselli

Volume 11, Issue 2, 2021

Published on: 10 January, 2020

Page: [182 - 192] Pages: 11

DOI: 10.2174/2210315510666200110154053

Price: $65

Abstract

Background: Infectious diseases are responsible for millions of deaths a year worldwide. Antibiotics, which have saved so many lives and improved life expectancy, may become ineffective due to a worrying increase in bacterial resistance. Some of the appropriate actions that could be initiated to address this problem are to develop and search for new antimicrobial substances from medicinal plants, and combine antibiotics with antimicrobials agents isolated from a reservoir of bioactive natural products.

Objectives: The purpose of this work was to study the chemical composition of the essential oil and hydrosol extract of Plumbago europaea, to evaluate their in-vitro antimicrobial activities and evaluate in-vitro combinatory antimicrobial effect of hydrosol extract with Gentamicin and Amphotericin B against a large panel of microorganisms in an effort to reduce their minimum effective dose and minimizing their side effects.

Methods: The essential oil and hydrosol extract obtained from the roots of Plumbago europaea were analyzed by GC/MS and tested for their antibacterial and antifungal activities against twelve different strains of microorganisms. The effectiveness, in-vitro, of the association between the hydrosol extract and both Gentamicin and Amphotericin B was also investigated using the checkerboard method.

Results: The obtained results revealed that nine and four components, representing for 92.4% and 97.4% of the total essential oil and hydrosol extract composition were identified, respectively and hydrosol extract was more active than the essential oil against all screened microorganisms, with interesting MIC values (19 μg/mL). An important effect of hydrosol extract was obtained in decreasing the MIC of Gentamicin and Amphotericin B in all tested combinations.

Conclusion: The in-vitro combination of the hydrosol extract with Gentamicin and Amphotericin B led to substantial MIC reduction against all tested microorganisms. This combination can help to reduce the minimum effective dose of antimicrobial drugs used, which may help to decrease their side effects; and deliver these medicines with similar potency.

Keywords: Plumbago europaea, plumbagin, hydrosol extract, antimicrobial substances, bioactive natural product, antimicrobial drugs.

Graphical Abstract

[1]
Haller, B.F. Medicinal Plants: Can utilization and conservation coexist? Advances in Economic Botany, 1997, 12, i-104.
[2]
Bodeker, G.; Bhat, K.; Burley, J.; Vantomme, P. Medicinal plants for forest conservation and health care; Univeristy of Pennsylvania Press: Pennsylvania, 1997.
[3]
Hoareau, L.; DaSilva, E. J. Medicinal plants: a re-emerging health aid. Electron. J. Biotechnol., 1999, 2(2), 3-4.
[4]
Sharma, A.; Verma, R.; Ramteke, P. Antibacterial activity of some medicinal plants used by tribals against UTI causing pathogens. World Appl. Sci. J., 2009, 7(3), 332-339.
[5]
Eryigit, T.; Yildirim, B.; Ekici, K.; Çirka, M. Chemical composition, antimicrobial and antioxidant properties of Schinus molle L. essential oil from Turkey. J. Essent. Oil Bear Pl., 2017, 20(2), 570-577.
[http://dx.doi.org/10.1080/0972060X.2017.1304286]
[6]
Kalemba, D.; Kunicka, A. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem., 2003, 10(10), 813-829.
[http://dx.doi.org/10.2174/0929867033457719]
[7]
Ghannadi, A.; Bagherinejad, M.; Abedi, D.; Jalali, M.; Absalan, B.; Sadeghi, N. Antibacterial activity and composition of essential oils from Pelargonium graveolens L’Her and Vitex agnus-castus L. Iran. J. Microbiol., 2012, 4(4), 171-176.
[8]
Verma, R.S.; Pandey, V.; Padalia, R.C.; Saikia, D.; Krishna, B. Chemical composition and antimicrobial potential of aqueous distillate volatiles of Indian peppermint (Mentha piperita) and spearmint (Mentha spicata). J. Herbs Spices Med. Plants, 2011, 17(3), 258-267.
[http://dx.doi.org/10.1080/10496475.2011.591519]
[9]
Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M. Biological effects of essential oils-a review. Food Chem. Toxicol., 2008, 46(2), 446-475.
[http://dx.doi.org/10.1016/j.fct.2007.09.106]
[10]
Rosato, A.; Vitali, C.; Gallo, D.; Balenzano, L.; Mallamaci, R. The inhibition of Candida species by selected essential oils and their synergism with amphotericin B. Phytomedicine, 2008, 15(8), 635-638.
[http://dx.doi.org/10.1016/j.phymed.2008.05.001]
[11]
Chouhan, S.; Sharma, K.; Guleria, S. Antimicrobial activity of some essential oils-present status and future perspectives. Medicines (Basel), 2017, 4(3), 58.
[http://dx.doi.org/10.3390/medicines4030058]
[12]
Moon, T.; Wilkinson, J.; Cavanagh, H. Antibacterial activity of essential oils, hydrosols and plant extracts from Australian grown Lavandula spp. Int. J. Aromather., 2006, 16(1), 9-14.
[http://dx.doi.org/10.1016/j.ijat.2006.01.007]
[13]
Djabou, N.; Dib, M.E.A.; Tabti, B.; Costa, J.; Muselli, A. Chemical composition and antioxidant activity of hydrosol extracts obtained by liquid–liquid extraction (LLE) of Daucus muricatus L. J. Essent. Oil Res., 2014, 26(6), 393-399.
[http://dx.doi.org/10.1080/10412905.2014.964427]
[14]
Boyraz, N.; Ozcan, M. Antifungal effect of some spice hydrosols. Fitoterapia, 2005, 76(7-8), 661-665.
[http://dx.doi.org/10.1016/j.fitote.2005.08.016]
[15]
Belabbes, R.; Dib, M.E.A.; Djabou, N.; Ilias, F.; Tabti, B.; Costa, J.; Muselli, A. Chemical variability, antioxidant and antifungal activities of essential oils and hydrosol extract of Calendula arvensis L. from Western Algeria. Chem. Biodivers., 2017, 14(5)
[http://dx.doi.org/10.1002/cbdv.201600482]]
[16]
Dzotam, J.K.; Touani, F.K.; Kuete, V. Antibacterial activities of the methanol extracts of Canarium schweinfurthii and four other Cameroonian dietary plants against multi-drug resistant Gram-negative bacteria. Saudi J. Biol. Sci., 2016, 23(5), 565-570.
[http://dx.doi.org/10.1016/j.sjbs.2015.06.006]
[17]
Valle, D.L., Jr; Andrade, J.I.; Puzon, J.J.M.; Cabrera, E.C.; Rivera, W.L. Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria. Asian Pac. J. Trop. Biomed., 2015, 5(7), 532-540.
[http://dx.doi.org/10.1016/j.apjtb.2015.04.005]
[18]
Wikaningtyas, P.; Sukandar, E.Y. The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pac. J. Trop. Biomed., 2016, 6(1), 16-19.
[http://dx.doi.org/10.1016/j.apjtb.2015.08.003]
[19]
Carson, C.F.; Riley, T.V. Non-antibiotic therapies for infectious diseases. Commun. Dis. Intell. Q. Rep., 2003, 27(Suppl.), S143-S146.
[20]
Hemaiswarya, S.; Kruthiventi, A.K.; Doble, M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine, 2008, 15(8), 639-652.
[http://dx.doi.org/10.1016/j.phymed.2008.06.008]
[21]
Rosato, A.; Piarulli, M.; Corbo, F.; Muraglia, M.; Carone, A.; Vitali, M.E.; Vitali, C. In vitro synergistic antibacterial action of certain combinations of gentamicin and essential oils. Curr. Med. Chem., 2010, 17(28), 3289-3295.
[http://dx.doi.org/10.2174/092986710792231996]
[22]
Mahboubi, M.; Ghazian Bidgoli, F. In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine, 2010, 17(10), 771-774.
[http://dx.doi.org/10.1016/j.phymed.2010.01.016]
[23]
Naqishbandi, A. Iraqi J. Pharm Sci., 2009, 18, 54-59.
[24]
Jaradat, N.A.; Zaid, A.N.; Hussein, F. Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine. Chem. Biol. Technol. Agric., 2016, 3(1), 31.
[http://dx.doi.org/10.1186/s40538-016-0082-4]
[25]
Navaei, M.N.; Mirza, M.; Dini, M. Chemical composition of the essential oil of Plumbago europaea L. roots from Iran. Flavour Fragrance J., 2005, 20(2), 213-214.
[http://dx.doi.org/10.1002/ffj.1384]
[26]
Adams, R. Identification of essential oils by Capillary Gas Chromatography/Mass Spectroscopy; Allured Publ. Corp: Carol Stream, IL, 2001.
[27]
Kçnig, W.; Joulain, D.; Hochmuth, D. Terpenoids and Related Constituents of Essential Oils, Library of Mass Finder 2.1; Institute of Organic Chemistry, University of Hamburg: Hamburg, 2001.
[28]
Mc Lafferty, F.; Stauffer, D. Wiley Register of Mass Spectral Data,(6thedn). Mass spectrometry library search system bench-Top/PBM; Version, 1994, p. 3.
[29]
Standards, N.I.O. 1999. Available from: https://www.sec.gov/Archives/edgar/data/1736541/000119312518247401/d560276df1. htm
[30]
Performance standards for antimicrobial disk susceptibility tests: approved standards M7–A7 2006. Available from: https://clsi. org/standards/products/microbiology/documents/m02/
[31]
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : Approved standard, M27–A2 2012. Available from: https://clsi.org/media/1632/m07a10_sample.pdf
[32]
White, R.L.; Burgess, D.S.; Manduru, M.; Bosso, J.A. Comparison of three different in vitro methods of detecting synergy: Time-kill, checkerboard, and E test. Antimicrob. Agents Chemother., 1996, 40(8), 1914-1918.
[http://dx.doi.org/10.1128/AAC.40.8.1914]
[33]
Cheng, S.S.; Chua, M.T.; Chang, E.H.; Huang, C.G.; Chen, W.J.; Chang, S.T. Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at different ages. Bioresour. Technol., 2009, 100(1), 465-470.
[http://dx.doi.org/10.1016/j.biortech.2007.11.060]
[34]
Telci, I.; Demirtas, I.; Sahin, A. Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Ind. Crops Prod., 2009, 30(1), 126-130.
[http://dx.doi.org/10.1016/j.indcrop.2009.02.010]
[35]
Odds, F.C. Synergy, antagonism, and what the chequerboard puts between them. J. Antimicrob. Chemother., 2003, 52(1), 1-1.
[http://dx.doi.org/10.1093/jac/dkg301]
[36]
Mallavadhani, U.; Sahu, G.; Muralidhar, J. Screening of Plumbago species for the bio-active marker plumbagin. Pharm. Biol., 2002, 40(7), 508-511.
[http://dx.doi.org/10.1076/phbi.40.7.508.14685]
[37]
Veluri, R.; Diwan, P. Phytochemical and Pharmacological aspects of Plumbago zeylanica. Indian Drugs, 1999, 36(12), 724-730.
[38]
Padhye, S.; Dandawate, P.; Yusufi, M.; Ahmad, A.; Sarkar, F.H. Perspectives on medicinal properties of plumbagin and its analogs. Med. Res. Rev., 2012, 32(6), 1131-1158.
[http://dx.doi.org/10.1002/med.20235]
[39]
Mabona, U.; Viljoen, A.; Shikanga, E.; Marston, A.; Van Vuuren, S. Antimicrobial activity of southern African medicinal plants with dermatological relevance: From an ethnopharmacological screening approach, to combination studies and the isolation of a bioactive compound. J. Ethnopharmacol., 2013, 148(1), 45-55.
[http://dx.doi.org/10.1016/j.jep.2013.03.056]
[40]
de Paiva, S.R.; Figueiredo, M.R.; Aragão, T.V.; Kaplan, M.A.C. Antimicrobial activity in vitro of plumbagin isolated from Plumbago species. Mem. Inst. Oswaldo Cruz, 2003, 98(7), 959-961.
[http://dx.doi.org/10.1590/S0074-02762003000700017]
[41]
Jeyachandran, R.; Mahesh, A.; Cindrella, L.; Sudhakar, S.; Pazhanichamy, K. Antibacterial activity of Plumbagin and root extracts of Plumbago zeylanica. Acta Biol. Cracov. Ser.; Bot., 2009, 51(1), 17-22.
[42]
Nair, S.V.; Baranwal, G.; Chatterjee, M.; Sachu, A.; Vasudevan, A.K.; Bose, C.; Banerji, A.; Biswas, R. Antimicrobial activity of plumbagin, a naturally occurring naphthoquinone from Plumbago rosea, against Staphylococcus aureus and Candida albicans. Int. J. Med. Microbiol., 2016, 306(4), 237-248.
[http://dx.doi.org/10.1016/j.ijmm.2016.05.004]
[43]
Carson, C.F.; Riley, T.V. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J. Appl. Bacteriol., 1995, 78(3), 264-269.
[http://dx.doi.org/10.1111/j.1365-2672.1995.tb05025.x]
[44]
Asche, C. Antitumour quinones. Mini Rev. Med. Chem., 2005, 5(5), 449-467.
[http://dx.doi.org/10.2174/1389557053765556]
[45]
Koch, A.; Cox, H.; Mizrahi, V. Drug-resistant tuberculosis: challenges and opportunities for diagnosis and treatment. Curr. Opin. Pharmacol., 2018, 42, 7-15.
[http://dx.doi.org/10.1016/j.coph.2018.05.013]
[46]
Lewis, K. Antibiotics: Recover the lost art of drug discovery. Nature, 2012, 485(7399), 439-440.
[http://dx.doi.org/10.1038/485439a]
[47]
Organization, W.H. Antibacterial agents in clinical development: an analysis of the antibacterial clinical development pipeline, including tuberculosis; World Health Organization 2017. Available from: https://www.who.int/medicines/areas/rational_use/antibacteri al_agents_clinical_development/en/
[48]
Langeveld, W.T.; Veldhuizen, E.J.; Burt, S.A. Synergy between essential oil components and antibiotics: a review. Crit. Rev. Microbiol., 2014, 40(1), 76-94.
[http://dx.doi.org/10.3109/1040841X.2013.763219]
[49]
Fernandes, T.G.; de Mesquita, A.R.C.; Randau, K.P.; Franchitti, A.A.; Ximenes, E.A. In-vitro synergistic effect of Psidium guineense (Swartz) in combination with antimicrobial agents against Swartz) in combination with antimicrobial agents against Sci. World J., 2012, 2012
[50]
Laniado-Laborín, R.; Cabrales-Vargas, M.N. Amphotericin B: side effects and toxicity. Rev. Iberoam. Micol., 2009, 26(4), 223-227.
[http://dx.doi.org/10.1016/j.riam.2009.06.003]
[51]
Dupont, B.; Dromer, F.; Improvisi, L. The problem of resistance to azoles in Candida. J. Mycol. Med., 1996, 6(2), 12-19.
[52]
Serra, E.; Hidalgo-Bastida, L.A.; Verran, J.; Williams, D.; Malic, S. Antifungal activity of commercial essential oils and biocides against Candida albicans. Pathogens, 2018, 7(1), 15.
[http://dx.doi.org/10.3390/pathogens7010015]
[53]
Terlecka, J.A.; du Cros, P.A.; Orla Morrissey, C.; Spelman, D. Rapid differentiation of Candida albicans from non-albicans species by germ tube test directly from BacTAlert blood culture bottles. Mycoses, 2007, 50(1), 48-51.
[http://dx.doi.org/10.1111/j.1439-0507.2006.01307.x]
[54]
Saral, R. Candida and Aspergillus infections in immunocompromised patients: an overview. Rev. Infect. Dis., 1991, 13(3), 487-492.
[http://dx.doi.org/10.1093/clinids/13.3.487]
[55]
Karkowska-Kuleta, J.; Rapala-Kozik, M.; Kozik, A. Fungi pathogenic to humans: molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochim. Pol., 2009, 56(2), 211-224.
[http://dx.doi.org/10.18388/abp.2009_2452]
[56]
Ullmann, A.J.; Akova, M.; Herbrecht, R.; Viscoli, C.; Arendrup, M.C.; Arikan-Akdagli, S.; Bassetti, M.; Bille, J.; Calandra, T.; Castagnola, E.; Cornely, O.A.; Donnelly, J.P.; Garbino, J.; Groll, A.H.; Hope, W.W.; Jensen, H.E.; Kullberg, B.J.; Lass-Flörl, C.; Lortholary, O.; Meersseman, W.; Petrikkos, G.; Richardson, M.D.; Roilides, E.; Verweij, P.E.; Cuenca-Estrella, M. ESCMID Fungal Infection Study Group. ESCMID* guideline for the diagnosis and management of Candida diseases 2012: adults with haematological malignancies and after haematopoietic stem cell transplantation (HCT). Clin. Microbiol. Infect., 2012, 18(Suppl. 7), 53-67.
[http://dx.doi.org/10.1111/1469-0691.12041]

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