Background: The exact relationship between the different TCF7L2 gene polymorphisms
and the development of diabetic nephropathy (DN) remains unclear.
Objective: To investigate the association of TCF7L2 rs12255372 (G/T) gene polymorphism and diabetic
nephropathy (DN) in patients with type 2 diabetes (T2D).
Methods: 100 patients with T2D (50 patients without DN and 50 patients with DN) and 50 age and
sex-matched healthy controls (HC) were enrolled in the study. Genotyping for the rs12255372 (G>T)
polymorphism in the TCF7L2 gene was performed by real-time PCR.
Results: The rs12255372 polymorphism showed a statistically significant difference between HC and
patients with and without DN in both the genotype and allele frequency. However, the rs12255372
polymorphism genotype or allele frequency was not statistically different between patients with DN
and those patients without DN. The G allele was found to be higher in patients and the T allele was
higher in HC suggesting that the G allele was the risk allele for developing T2D & DN and that the T
allele was protective.
Conclusion: rs12255372 TCF7L2 gene polymorphism was strongly associated with type 2 diabetes
mellitus and DN. The association between rs12255372 polymorphism and DN was a mere reflection of
a complicated diabetes mellitus rather than a direct independent association.