Background: In this project, the growth and volatile metabolites profiles of Escherichia
coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical,
nanoparticle and ultra-highly diluted compounds.
Materials and Methods: The treatments were done for 12000 life cycles using silver nanoparticles
(AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites
analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated
that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle
condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later.
Also, with the continuous usage of these materials, no more bacterial growth was observed.
Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate,
Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol.
The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde,
2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were
common and produced by both organisms.
Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample
is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols
in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).