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Current Bioinformatics

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ISSN (Print): 1574-8936
ISSN (Online): 2212-392X

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Research Article

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

Author(s): Li Li, Jiemin Li, Jian Sun*, Ping Yi, Changbao Li, Zhugui Zhou, Ming Xin, Jinfeng Sheng, Liang Shuai, Zhichun Li, Dongning Ling, Xuemei He, Fengjin Zheng, Guoming Liu and Yayuan Tang

Volume 14, Issue 7, 2019

Page: [649 - 657] Pages: 9

DOI: 10.2174/1574893614666190503162645

Price: $65

Abstract

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit.

Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature.

Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit.

Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage.

Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

Keywords: Phospholipase D, postharvest longan, 2-butanol, senescence, gene expression, inhibitor.

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